1. Identification and monitoring of effector and regulatory T cells during experimental arthritis based on differential expression of CD25 and CD134
- Author
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Nolte-'t Hoen, E.N.M., Boot, E.P.J., Wagenaar-Hilbers, J.P.A., Bilsen, J.H.M. van, Arkesteijn, G.J.A., Storm, G., Everse, L.A., Eden, W. van, Wauben, M.H.M., and TNO Kwaliteit van Leven
- Subjects
interleukin 2 receptor alpha ,Male ,Immune regulation ,CD134 antigen ,phenotype ,animal experiment ,transcription factor FOXP3 ,memory T lymphocyte ,chemical and pharmacologic phenomena ,CD25+ T lymphocyte ,animal cell ,protein localization ,T-Lymphocytes, Regulatory ,regulatory T lymphocyte ,FoxP3 ,Animals ,controlled study ,rat ,Biology ,Lymph nodes ,protein expression ,CD4+ T lymphocyte ,nonhuman ,Rattus ,animal model ,article ,Interleukin-2 Receptor alpha Subunit ,hemic and immune systems ,Forkhead Transcription Factors ,lymph node ,protein function ,Receptors, OX40 ,Arthritis, Experimental ,cell activation ,Rats ,Kinetics ,cell differentiation ,Disease Models, Animal ,cell proliferation ,arthritis ,priority journal ,protein analysis ,Rats, Inbred Lew ,protein isolation ,Disease Progression ,effector cell - Abstract
Major problems in the analysis of CD4+ effector cell and regulatory T cell (Treg) populations in an activated immune system are caused by the facts that both cell types can express CD25 and that the discriminatory marker forkhead box p3 can only be analyzed in nonviable (permeabilized) cells. Here, we show that CD134 (OX40) can be used as a discriminatory marker combined with CD25 to isolate and characterize viable CD4+ effector cells and Tregs. Before and during adjuvant arthritis in rats, coexpression of CD134 and CD25 identified activated Tregs consistently, as these T cells proliferated poorly to disease-associated antigens and were suppressive in vitro and in vivo. Depending on the time of isolation and location, CD4+ T cell populations expressing CD134 or CD25 contained effector/memory T cells. Analysis of the function, phenotype, and amount of the CD4+ T cell subsets in different lymph node stations revealed spatiotemporal differences in effector cell and Treg compartments during experimental arthritis. © Society for Leukocyte Biology.
- Published
- 2008