1. The use of quorum sensing to improve vaccine immune response.
- Author
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Sturbelle RT, Conceição RC, Da Rosa MC, Roos TB, Dummer L, and Leite FP
- Subjects
- Animals, Antibodies, Bacterial blood, Antibodies, Bacterial immunology, Bacterial Toxins genetics, Bacterial Toxins immunology, Enterotoxins genetics, Enterotoxins immunology, Escherichia coli Proteins genetics, Escherichia coli Proteins immunology, Escherichia coli Vaccines genetics, Female, Fimbriae, Bacterial genetics, Mice, Quorum Sensing genetics, Swine, Animal Diseases immunology, Animal Diseases prevention & control, Enterotoxigenic Escherichia coli immunology, Escherichia coli Infections veterinary, Escherichia coli Vaccines immunology, Quorum Sensing immunology
- Abstract
Enterotoxigenic Escherichia coli (ETEC) infection is an important cause of diarrhea in both newborn and post-weaning pigs, it is also responsible for economic losses on farms worldwide. Vaccines that use ETEC virulence factors have been well documented, and several vaccines containing inactivated bacteria with protective antigens, or purified (isolated) antigens are available on the market. Vaccination of pregnant sows is widely seen as an effective strategy for the control of the disease. Yet these vaccines very often do not lead to efficient protection. In this study, we produced an ETEC bacterin with the use of quorum sensing (QS), and observed a significant expression of F4 adhesin, and heat-labile toxin (LT) in the cultures when compared to the controls. Mice, and pigs vaccinated with the QS bacterin demonstrated higher antibody titers against these antigens when compared with commercial and control bacterin. Our results suggest that the system might bring promising improvements in ETEC bacterin efficacy., (Copyright © 2013 Elsevier Ltd. All rights reserved.)
- Published
- 2013
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