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This study compared the detailed mineral profiles of milk from purebred Holstein (HO) and four generations (F1 to F4) of crossbred cows (CR) derived from a 3-breed rotational crossbreeding system involving Viking Red (VR) and Montbéliarde (MO) sires. Purebred and CR were kept and fed together in the same herd. Individual milk samples were collected once per cow from 120 multiparous cows (40 HO and 20 for each generation of CR from F1 to F4). The milk samples were analysed for macrominerals (Na, Mg, P, S, K, Ca), essential (Cr, Mn, Fe, Cu, Zn, Mo), and environmental microminerals (Li, B, Ba) using inductively coupled plasma-optical emission spectrometry. The coefficients of variation ranged from 11 to 28% for macrominerals and from 17 to 40% for microminerals. The mineral composition of milk from purebred HO and CR were mostly comparable. Conversely, it differed in different breed combinations of CR: VR-sired cows had the greatest macromineral contents, MO-sired cows the lowest, while HO-sired CR was intermediate. The milk from MO-sired crossbreds had lower contents of some microminerals (Mo, Zn, and Ba), while the milk from HO-sired crossbreds had greater contents (Fe and Mo). We conclude that the mineral profile of milk of CR derived from different combinations of VR, MO and HO breeds was comparable to that of milk from HO cows kept in the same herd, and that the variations in the macro- and micromineral profiles were greater among CR than between purebreds and crossbreds.Highlights The mineral composition affects the nutritional and technological properties of milk, and it is influenced by breed effect. Macro- and micromineral compositions of milk from Holstein and crossbred cows from a 3-breed rotational crossbreeding system were mostly comparable. Crossbred cows of different generations showed great variations in the macro- and micromineral profiles.

Minerals are limiting factors in animal production, and the knowledge of mineral requirements for livestock is crucial to the success of a commercial enterprise. Hair sheep may have different mineral requirements than those presents by the international committees. A study was carried to evaluate the net calcium (Ca), phosphorus (P), magnesium (Mg), sodium (Na), potassium (K), zinc (Zn), iron (Fe), manganese (Mn) and copper (Cu) requirements for the growth and maintenance of Brazilian Somali lambs. A total of 48 hair lambs (13.5±1.8 kg) aged 60±15 days were allocated to individual pens. Eight animals were slaughtered at the beginning of the experiment to serve as a reference group to estimate initial empty BW (EBW) and initial body composition. The remaining lambs (n=40) were assigned to a completely randomized design with eight replications in five levels of metabolizable energy (ME; 4.93, 8.65, 9.41, 10.12 and 11.24 MJ/kg DM). When the lambs of a given treatment reached an average BW of 28 kg, they were slaughtered. Initial body composition was used to calculate the retention of minerals. Mineral body composition was fit using a logarithmic equation in the form of a nonlinear model. The maintenance requirements were estimated from regressions of mineral retention in the empty body on mineral intake. The body mineral concentration decreased in lambs with a BW ranging from 15 to 30 kg. The net mineral requirements (100 g/day of average daily gain (ADG)) decreased from 0.52 to 0.51 g for Ca, 0.28 to 0.23 g for P, 0.02 to 0.02 g for Mg, 0.09 to 0.08 g for Na, 0.11 to 0.09 g for K, 1.30 to 1.08 mg for Zn, 3.77 to 3.22 mg for Fe, 0.08 to 0.06 mg for Mn and 0.09 to 0.08 mg for Cu when BW increased from 15 to 30 kg. The daily net requirements for maintenance per kilogram of BW were 30.13 mg of Ca, 27.58 mg of P, 1.26 mg of Mg, 4.12 mg of Na, 8.11 mg of K, 0.133 mg of Zn, 0.271 mg of Fe, 0.002 mg of Mn and 0.014 mg of Cu. The results of this study indicate that the net mineral requirements for weight gain and maintenance in Brazilian Somali lambs are different than the values that are commonly recommended by the main evaluation systems for feed and nutritional requirements for sheep. These results for the nutritional requirements of minerals may help to optimize mineral supply for hair sheep.

ABSTRACT This experiment was conducted to investigate the effects available phosphorus levels and dicalcium phosphate particle size on the live performance, and egg parameters and bone parameters of Japanese quails in lay. The experimental diets were based on corn and soybean meal and formulated to contain 2900 kcal metabolizable energy (ME)kg-1, 200 g kg-1 crude protein (CP) and 30.5 g kg-1 calcium (Ca). Feeds were supplied ad libitum. Laying Japanese quails (n=720, 20-wk-old)were distributed in a completely randomized design in a 5 x 2 factorial arrangement, with five levels of available phosphorus (avP; 1.0, 2.0, 3.0, 4.0, 5.0 g kg-1) and two dicalcium phosphate particle sizes (fine orcoarse), totaling ten treatments with eight replicates of nine birds each. Egg laying rate, feed intake per bird per day, egg weight, egg mass, feed conversion ratio per egg mass and per dozen eggs, eggshell, yolk and albumen relative and absolute weights, as well as bone ash, phosphorus, calcium, and magnesium contents were evaluated. Based on the results, avP recommendations for 20- to 32-wk-old laying Japanese quails varies according with phosphate source particle size and the dietary avP levels of 4.40 g kg-1 and 3.85 g kg-1avP levels when using fine and coarse particle dicalcium phosphate, respectively, yielded the best results.

This experiment was carried out with the objective of determining the macrominerals (Ca, P, Mg, K and Na) requirements of Nellore steers under grazing. Twenty four Nellore steers (371 ± 14 kg of BW and 26 mo old) were used. Four steers were slaughtered at the beginning of the experiment (reference group), serving as a reference in subsequent study. The remaining 20 animals were weighed and distributed into a completely randomized design with four supplementation levels offer: 0.0 (mineral mixture - control), 0.3, 0.6 and 0.9% of BW, with five replications. The supplements, based on ground corn, soybean meal and/or urea, were previously balanced to achieve an average daily gain of 350, 650 and 850g, respectively, for the different supplementation levels offer. The contents of macrominerals retained in the animal body were determined by regression equations of the macrominerals body content logarithm in function of the empty body weight logarithm (EBW). Net macrominerals requirements for a gain of 1kg of EBW were obtained using the equation Y'= b.10ª.Xb-1, with a and b, respectively, the intercept and the regression coefficient of the prediction equations of macrominerals in the animal body contents for each macromineral considered. The concentrations of all macrominerals, in the empty body weight and gain of the empty body weight, decreased with the increase in the body weight. Total calcium and phosphorus dietary requirements are higher than those recommended in the literature.Com o objetivo de determinar as exigências de macrominerais (Ca, P, Mg, K e Na) de bovinos Nelore castrados sob pastejo, foi conduzido um experimento com 24 novilhos da raça Nelore, castrados, com peso inicial de 371 ± 14 kg e 26 meses de idade. Quatro novilhos foram abatidos no início do experimento (grupo referência) para servir de referência nos estudos subsequentes. Os animais restantes (20) foram pesados e distribuídos em delineamento inteiramente casualizado com quatro níveis de suplementação: 0,0 (mistura mineral - controle); 0,3; 0,6 e 0,9% do peso corporal e cinco repetições. Os suplementos, à base de milho, farelo de soja e/ou uréia, foram balanceados previamente para promover ganhos médios diários de 350, 650 e 850 g, respectivamente. Os conteúdos de macrominerais retidos no corpo animal foram estimados por meio de equações de regressão do logaritmo do conteúdo corporal dos macrominerais, em função do logaritmo do peso de corpo vazio (PCVZ). As exigências líquidas dos macrominerais para ganho de 1 kg de PCVZ foram obtidas utilizando-se a equação Y' = b.10ª. Xb-1, em que a e b são o intercepto e o coeficiente de regressão, respectivamente, das equações de predição dos conteúdos corporais de cada macromineral considerado. As concentrações de todos os macrosminerais estudados, no corpo vazio e no ganho de corpo vazio, diminuíram com o aumento do peso vivo. As exigências dietéticas totais de cálcio e fósforo são superiores às recomendadas na literatura.

Com o objetivo de determinar as exigências dos macroelementos minerais (Ca, P, Mg, K e Na) de zebuínos, foi conduzido um experimento no Departamento de Zootecnia da Universidade Federal de Viçosa, utilizando-se 16 novilhos castrados, com peso vivo médio inicial de 270 kg, mantidos em confinamento. Quatro animais foram abatidos, após o período de adaptação, para servirem como referência, e os doze restantes foram distribuídos em três tratamentos com diferentes níveis de concentrado nas dietas (5, 35 e 65%), na base da matéria seca (MS) total, em delineamento inteiramente casualizado, com quatro repetições. As dietas foram formuladas de forma a serem isoprotéicas. O volumoso foi constituído de pré-secado de capim-braquiária (Brachiaria brizantha) e de capim-tifton 85 (Cynodon sp.). Os conteúdos de macroelementos minerais retidos no corpo foram estimados por meio de equações de regressão do logaritmo do conteúdo corporal dos macroelementos minerais, em função do logaritmo do peso de corpo vazio (PCVZ). As exigências líquidas dos macroelementos minerais, para ganho de 1 kg de PCVZ, foram obtidas utilizando-se a equação Y' = b.10ª. Xb-1, sendo a e b o intercepto e o coeficiente de regressão, respectivamente, das equações de predição dos conteúdos corporais de cada macroelemento mineral considerado. Verificou-se diminuição nas concentrações de todos os macroelementos minerais estudados, no corpo vazio e no ganho de corpo vazio, com a elevação do peso vivo. As relações obtidas para g Ca/100g de proteína retida e g P/100 g de proteína retida foram, respectivamente, 10,92 e 5,26.With the objective to determine the macrominerals (Ca, P, Mg, Na, K) requirements of zebu cattle, a trial involving sixteen zebu steers with initial live weight of 270 kg was conducted. Four steers were slaughtered after the adaptation period of the trial, performing the reference group, and the remaining were uniformly allotted to a complete randomized design, in three treatments, with different levels of concentrate in the diets (5, 35, 65 %, in the total dry matter basis). The diets were formulated to be isoproteic. The roughage used was Brachiaria brizantha and Cynodon sp. haylage. The macrominerals contents retained in the body were estimated by regression equations of the logarithm of the macrominerals contents in the body, in function of the logarithm of the empty body weight (EBW). By deriving the prediction equations of macrominerals body content, in function of the logarithm of EBW, it was obtained the net macrominerals requirements for gain of 1 kg EBW, through the equation Y`= b.10ª.Xb-1, being "a" and "b" intercept and the regression coefficient, respectively, of the prediction equations of macrominerals contents in the body. It was observed a decrease in the concentrations of all the macromineral studied in the empty body and in the gain of empty body as the live weight increased. The relationships obtained for g Ca/100g of retained protein and g P/100 g of retained protein were 10.92 and 5.26, respectively.

Foram utilizados 50 novilhos F1 Limousin x Nelore inteiros, alocados em dez tratamentos, com cinco níveis de concentrado (25; 37,5; 50; 62,5; e 75%) e duas formas de balanceamento protéico da dieta (uma isoprotéica com 12% de proteína bruta [PB] e outra variando proteína com energia). O volumoso utilizado foi feno de capim-Coastcross (Cynodon dactylon). Após o abate, todas as partes do corpo do animal foram pesadas e amostradas. As amostras foram secas, pré-desengorduradas com éter, moídas e foram determinados os teores de macroelementos minerais. O conteúdo corporal de Ca, P, Na, K e Mg foi determinado em função das concentrações destes nas várias partes do corpo. O conteúdo de macroelementos minerais retidos no corpo foi estimado por meio de equações de regressão do logaritmo do conteúdo corporal dos macroelementos minerais em função do logaritmo do peso de corpo vazio (PCVZ). As exigências líquidas dos macroelementos minerais, para ganho de 1 kg de PCVZ, foram obtidas utilizando a equação Y' = b. 10ª. Xb-1, sendo a e b o intercepto e o coeficiente de regressão, respectivamente, das equações de predição dos conteúdos corporais dos macroelementos minerais. Houve diminuição nas concentrações dos cinco macroelementos estudados no corpo vazio e no ganho de corpo vazio, com a elevação do peso vivo. As relações g Ca/100 g de proteína retida e g P/100 g de proteína retida foram iguais a 8,70 e 3,46, respectivamente.Fifty F1 Limousin x Nellore bulls were allotted to ten treatments, with five concentrate levels (25; 37.5; 50; 62.5 e 75%) and two diet protein balance methods (one isoprotein and the other changing protein as diet energy changed). The roughage used was Coastcross grass hay (Cynodon dactylon). After the slaughter, all animal body parts were weighted and sampled. The samples were dried, most of the fat was extracted with ether, grinded, and the concentrations of macrominerals were determined. Ca, P, Na, K and Mg contents in the body were determined as function of their concentrations in the several parts of the body. Macrominerals contents retained in the body were determined by regression equations of the logarithm of macrominerals contents in the body as function of the logarithm of empty body weight (EBW). By deriving the prediction equations of macrominerals body content as function of the logarithm of EBW, it was obtained the net macrominerals requirements, for gains of 1 kg EBW, through the equation Y' = b. 10ª. Xb-1, being "a" and "b" intercept and the regression coefficient, respectively, of the prediction equations of macrominerals contents in the body. There was a decrease in the empty body and in the gain of empty body concentrations for all macrominerals studied, with live weight increase. The relationship g Ca/100 g of retained protein and g P/100 g of retained protein were 8.70 and 3.46, respectively.

A composição corporal e as exigências de macroelementos minerais (Ca, P, Mg, Na e K) para ganho de peso de bovinos de quatro raças zebuínas (Gir, Guzerá, Mocho-Tabapuã e Nelore) foram estimadas com 63 animais não-castrados, com idade média de 24 meses e 366 kg de PVinicial. Dezesseis animais das raças Gir, Guzerá e Mocho-Tabapuã e 15 da raça Nelore foram usados. Quinze animais, quatro das raças Gir, Guzerá e Mocho-Tabapuã e três da Nelore, foram abatidos ao início do estudo e os demais, distribuídos em três grupos, com três animais de cada raça e alimentados individualmente, ad libitum, com ração contendo 50% de concentrado na matéria seca, até atingirem peso de abate de 405, 450 e 500 kg, respectivamente. Equações de regressão do logaritmo do conteúdo corporal de macroelementos minerais, em função do logaritmo do corpo vazio (PCV), foram ajustadas para estimar os composição corporal dos constituintes para qualquer PCV, dentro do intervalo incluído na pesquisa (250 a 450kg). Pela derivação destas equações, a composição do ganho de PCV foi obtida. Animais das quatro raças não diferiram quanto à composição corporal de macroelementos minerais e às exigências líquidas para ganho de peso. Houve decréscimo na concentração dos cinco macroelementos no PCV e no peso ganho, com a elevação do PCV. As exigências líquidas estimadas para ganho de PCV, para os cinco macrominerais, foram inferiores aos valores fixos sugeridos pelo ARC (1980). Os requerimentos líquidos estimados de Ca e P para ganho de peso de um bovino não-castrado de 400 kg PV foram 16 e 24% inferiores, respectivamente, aos propostos pelo AFRC (1991).The body composition and macrominerals requirements for weight gain of four Zebu breeds (Gyr, Guzera, Mocho-Tabapuã and Nellore) were estimated using 63 bulls averaging 24 months of age and 366 kg initial LW. Sixteen animals from Gir, Guzera e Mocho-Tabapuã breeds and 15 from Nellore breed. Fifteen animals, four animals from Gyr, Guzera and Mocho-Tabapuã breeds and three from Nellore breed, were slaughtered at the beginning of the trial. The remaining animals were distributed into three groups, containing three animals from each breed, which were individually fed a diet containing 50% concentrate as dry matter base, to reach the target live weight of 405, 450 and 500 kg, respectively. Regression of the log the of body composition of macrominerals, on the log of empty body weight (EBW), was fitted to estimate the total body composition of those constituents for any EBW inside the range included in this research (250 to 450 kg). By derivation of those regression, the composition of gain was rejusted. The animals from the four breeds did not differ regarding to body composition of macroelements minerals, as for the net requirements for weight gain. There were decreases in the concentration of the five macrominerals in the EBW and in the weight gain, as the EBW increased. The estimated net requirements for EBW gain, for the five macrominerals were lower as compared to the fixed values suggested by ARC (1980). The estimated Ca and P net requirements for weight gain of a 400 kg LW bulls were 16 and 24% lower, respectively, to the proposed by the AFRC (1991).

The chemical composition and in vitro dry matter digestibility of 45 samples of different species of lichen are reported. Mean content (g/100 g dry matter) of the main nutrients was: crude protein 4.2, crude fat 3.2, crude fibre 16.6, ash 1.9, Ca 0.15, P 0.09, Mg 0.05, K 0.13, Na 0.035, S 0.07. The content of microminerals (mg/kg dry matter) was: Cu 2.5, Mo 0.11, Zn 27.2, Se 0.12, Fe 898, Mn 154. The mean in vitro dry matter digestibility was 35%. However, the in vitro method do underestimate the dry matter digestibility of lichens. Stereocaulon spp. showed higher levels of crude protein, P, S, Cu and Mo than Cetraria spp. and Cladonia spp. Cetraria nivalis showed higher digestibility and contained more NFE, ash, Ca, Mg, but less crude fibre than Cladonia stellaris. Lichens contained less amounts of most nutrients compared with grasses (Fig. 1), exept for crude fat, NFE, Se and Fe. Kjemisk innhald og in vitro fordøyelsesgrad av lav. Abstract in Norwegian / Samandrag: Kjemisk innhald og in vitro fordøyelsesgrad av tørrstoffet er bestemt i 45 prøver av beitelav frå to stader i Sør-Noreg. Middel innhald (g/100g tørrstoff) av følgjande næringsstoff var: protein 4.2, feitt 3.2, trevlar 16.6, oske 1.9, kalsium 0.15, fosfor 0.09, magnesium 0.05, kalium 0.13, natrium 0.035, svovel 0.07. Innhaldet (mg/kg tørrstoff) av mikronæringsstoffa var: kopar 2.5, molybden 0.11, sink 27.2, selen 0.12, jern 898 og mangan 154. Den midlare fordøyelsesgraden av tørrstoffet i lav-prøvene var 35%, men in vitro fordøyelsesanalyser undervurderer fordøyelsesgraden av lav. Det var ein stor variasjon mellom dei ulike lavartane for dei fleste næringsstoffa og fordøyelsesgraden. Stereocaulon spp. inneheldt meir protein, fosfor, svovel, kopar og molybden enn Cetraria spp. og Cladonia spp. Gulskinn hadde høgare fordøyelsesgrad, og innehaldet av NFE, oske, kalsium og magnesium var høgre enn i kvitkrull, medan trevleinnhaldet var størst i kvitkrull. Lav inneheldt mindre av dei fleste næringsstoffa samanlikna med gras, unnateke feitt, NFE, selen og jern. Jäkälien kemiallinen sisältö ja in vitro sulatuksen aste. Abstract in Finnish / Yhteenveto: Kuiva-aineen kemiallinen sisalto ja in vitro sulatusaste on mååråtty 45:ssa kokeessa tårkeistå laidunjåkålistå kahdella paikkakunnalla Etelå — Norjassa. Keskimååråinen sisalto (g/100 g kuiva-ainetta) seuraavissa ravintoaineissa oli: valkuaisainetta 4.2, rasvaa 3.2, kuituja 16.6, tuhkaa 1.9, kalsiumia 0.15, fosforia 0.09, magnesiumia 0.05, kaliumia 0.13, natriumia 0.035, rikkia 0.07. Hivenainepitoisuus (mg/kg kuiva-ainesta) oli: kuparia 2.5, molybdeenia 0.11, sinkkia 27.2, seleeniå 0.12, rautaa 898 ja mangaania 154. Kuiva-aineiden keskimååråinen sulatusaste jåkålåkokeissa oli 35%. Eri jåkålåajien vålillå oli suuri vaihtelu eisimmissa ravintoaineissa ja sulatusasteessa. Stereocaulon spp. sisålsi enemmån valkuaisainetta, fosforia, rikkiå kuparia ja typettomien uuteaineiden, molybdeeniå kuin Cetraria spp. ja Cladonia spp. Lapalumijåkålållå oli korkeampi sulatusaste, ja tuhkan, kalsiumin ja magnesiumin sisålto oli korkeampi kuin Cladonia alpestris-jäkälässä, mutta kuitusisålto oli korkein Cladonia alpestris-jäkälässä. Jåkålå sisålsi våhemmån useimmista ravintoaineista ruohoon verrattuna lukuunottamatta rasvaa, typettomiå uuteaineita, seleeniå ja rautaa.