1. Cdo Regulates Surface Expression of Kir2.1 K+ Channel in Myoblast Differentiation.
- Author
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Leem, Young-Eun, Jeong, Hyeon-Ju, Kim, Hyun-Ji, Koh, Jewoo, Kang, KyeongJin, Bae, Gyu-Un, Cho, Hana, and Kang, Jong-Sun
- Subjects
MYOBLASTS ,POTASSIUM channels ,HYPERPOLARIZATION (Cytology) ,CELL differentiation ,PROTEIN expression - Abstract
A potassium channel Kir2.1-associated membrane hyperpolarization is required for myogenic differentiation. However the molecular regulatory mechanisms modulating Kir2.1 channel activities in early stage of myogenesis are largely unknown. A cell surface protein, Cdo functions as a component of multiprotein cell surface complexes to promote myogenesis. In this study, we report that Cdo forms a complex with Kir2.1 during myogenic differentiation, and is required for the channel activity by enhancing the surface expression of Kir2.1 in the early stage of differentiation. The expression of a constitutively active form of the upstream kinase for p38MAPK, MKK6(EE) can restore Kir2.1 activities in Cdo-depleted C2C12 cells, while the treatment with a p38MAPK inhibitor, SB203580 exhibits a similar effect of Cdo depletion on Kir2.1 surface expression. Furthermore, Cdo
-/- primary myoblasts, which display a defective differentiation program, exhibit a defective Kir2.1 activity. Taken together, our results suggest that a promyogenic Cdo signaling is critical for Kir2.1 activities in the induction of myogenic differentiation. [ABSTRACT FROM AUTHOR]- Published
- 2016
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