1. Association of the Leptin Gene with Carcass Characteristics in Nellore Cattle.
- Author
-
de Oliveira, JoyceAzambuja, Cunha, CamilaMagalhães da, Crispim, Brunodo Amaral, Seno, Leonardode Oliveira, Fernandes, AlexandreRodrigo Mendes, Nogueira, Guilhermede Paula, and Grisolia, AlexéiaBarufatti
- Subjects
LEPTIN genetics ,CATTLE carcasses ,ARTIFICIAL selection of animals ,ANIMAL biotechnology ,EXONS (Genetics) ,GENETIC polymorphisms ,RESTRICTION fragment length polymorphisms ,CATTLE - Abstract
Advances in DNA technology have created biotechnological tools that can be used in animal selection and new strategies for increasing herd productivity and quality. The objective of the present work was to associate the genotypes of leptin gene exon 2 polymorphisms with productive traits in Nellore cattle. Blood was collected from Nellore males and PCR-RFLP reactions were performed with the restriction enzymesClaI andKpn2I. The gene frequencies resulting from digestion byClaI were 0.60 and 0.40 for allele A and T, respectively; the genotypic frequencies were AA = 0.20 and AT = 0.80. The gene frequencies from digestion byKpn2I were 0.81 for allele C and 0.194 for allele T; the genotypic frequencies were CC = 0.62 and CT = 0.38. The populations in both cases were not in Hardy-Weinberg equilibrium (p > 0.05), and the TT genotype was not found. Significant associations were noted between leptin gene exon 2 polymorphisms and five productive traits in Nellore cattle: carcass fat distribution, the intensity of red muscle coloration, pH, marbling, and post-slaughter fat thickness. [ABSTRACT FROM AUTHOR]
- Published
- 2013
- Full Text
- View/download PDF