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Start Over Author "Calierno, Mt" Topic angiogenesis inhibitors
2 results on '"Calierno, Mt"'

1. Antitumour and antiangiogenic activities of [Pt(O,O'-acac)(γ-acac)(DMS)] in a xenograft model of human renal cell carcinoma.

Author

Muscella A, Vetrugno C, Biagioni F, Calabriso N, Calierno MT, Fornai F, De Pascali SA, Marsigliante S, and Fanizzi FP

Subjects
Angiogenesis Inhibitors chemical synthesis, Angiogenesis Inhibitors chemistry, Animals, Antineoplastic Agents chemical synthesis, Antineoplastic Agents chemistry, Carcinoma, Renal Cell pathology, Cell Proliferation drug effects, Cell Survival drug effects, Dose-Response Relationship, Drug, Humans, Kidney Neoplasms pathology, Mice, Mice, Inbred BALB C, Mice, Nude, Neoplasms, Experimental drug therapy, Neoplasms, Experimental pathology, Organoplatinum Compounds chemical synthesis, Organoplatinum Compounds chemistry, Structure-Activity Relationship, Tumor Cells, Cultured, Angiogenesis Inhibitors pharmacology, Antineoplastic Agents pharmacology, Carcinoma, Renal Cell drug therapy, Kidney Neoplasms drug therapy, Organoplatinum Compounds pharmacology, Xenograft Model Antitumor Assays
Abstract

Background and Purpose: It is thought that the mechanism of action of anticancer chemotherapeutic agents is mainly due to a direct inhibition of tumour cell proliferation. In tumour specimens, the endothelial cell proliferation rate increases, suggesting that the therapeutic effects of anticancer agents could also be attributed to inhibition of tumour angiogenesis. Hence, we investigated the potential effects of [Pt(O,O'-acac)(γ-acac)(DMS)] ([Pt(DMS)]), a new platinum drug for non-genomic targets, on human renal carcinoma and compared them with those of the well-established anticancer drug, cisplatin., Experimental Approach: Tumour growth, tumour cell proliferation and microvessel density were investigated in a xenograft model of renal cell carcinoma, developed by injecting Caki-1 cells into BALB/c nude mice. The antiangiogenic potential of compounds was also investigated using HUVECs., Key Results: Treatment of the Caki-1 cells with cisplatin or [Pt(DMS)] resulted in a dose-dependent inhibition of cell survival, but the cytotoxicity of [Pt(DMS)] was approximately fivefold greater than that of cisplatin. [Pt(DMS)] was much more effective than cisplatin at inhibiting tumour growth, proliferation and angiogenesis in vivo, as well as migration, tube formation and MMP1, MMP2 and MMP9 secretion of endothelial cells in vitro. Whereas, cisplatin exerted a greater cytotoxic effect on HUVECs, but did not affect tube formation or the migration of endothelial cells. In addition, treatment of the xenograft mice with [Pt(DMS)] decreased VEGF, MMP1 and MMP2 expressions in tumours., Conclusions and Implications: The antiangiogenic and antitumour activities of [Pt(DMS)] provide a solid starting point for its validation as a suitable candidate for further pharmacological testing., (© 2016 The British Pharmacological Society.)

Published
2016
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2. Antitumour and antiangiogenic activities of [Pt(O,O'-acac)(γ-acac)(DMS)] in a xenograft model of human renal cell carcinoma

Author

Muscella, A, Vetrugno, C, Biagioni, F, Calabriso, N, Calierno, Mt, Fornai, Francesco, De Pascali, Sa, Marsigliante, S, Fanizzi, F. p., Muscella, Antonella, Vetrugno, Carla, Biagioni, F, Calabriso, Nadia, Calierno, M. T, Fornai, F, DE PASCALI, SANDRA ANGELICA, Marsigliante, Santo, and Fanizzi, Francesco Paolo

Subjects
RANDOMIZED PHASE-3 TRIAL, BREAST-CANCER CELLS, TUMOR ANGIOGENESIS, TESTICULAR CANCER, PLATINUM(II) COMPLEXES, ENDOTHELIAL-CELL, IN-VIVO, GROWTH, CISPLATIN, PATHWAY, Organoplatinum Compounds, Cell Survival, Mice, Nude, Angiogenesis Inhibitors, Antineoplastic Agents, renal cell carcinoma, VEGF, xenograft, [Pt(O,O′-acac)(γ-acac)(DMS)], MMPs matrix metalloprotease, Mice, Structure-Activity Relationship, Tumor Cells, Cultured, Animals, Humans, Carcinoma, Renal Cell, Cell Proliferation, Mice, Inbred BALB C, Dose-Response Relationship, Drug, Neoplasms, Experimental, Xenograft Model Antitumor Assays, Research Papers, Kidney Neoplasms
Abstract

BACKGROUND AND PURPOSE It is thought that the mechanism of action of anticancer chemotherapeutic agents is mainly due to a direct inhibition of tumour cell proliferation. In tumour specimens, the endothelial cell proliferation rate increases, suggesting that the therapeutic effects of anticancer agents could also be attributed to inhibition of tumour angiogenesis. Hence, we investigated the potential effects of [Pt (O,O′-acac)(γ-acac)(DMS)] ([Pt(DMS)]), a new platinum drug for non-genomic targets, on human renal carcinoma and compared them with those of the well-established anticancer drug, cisplatin. EXPERIMENTAL APPROACH Tumour growth, tumour cell proliferation and microvessel density were investigated in a xenograft model of renal cell carcinoma, developed by injecting Caki-1 cells into BALB/c nude mice. The antiangiogenic potential of compounds was also investigated using HUVECs. KEY RESULTS Treatment of the Caki-1 cells with cisplatin or [Pt(DMS)] resulted in a dose-dependent inhibition of cell survival, but the cytotoxicity of [Pt(DMS)] was approximately fivefold greater than that of cisplatin. [Pt(DMS)] was much more effective than cisplatin at inhibiting tumour growth, proliferation and angiogenesis in vivo, as well as migration, tube formation and MMP1, MMP2 and MMP9 secretion of endothelial cells in vitro. Whereas, cisplatin exerted a greater cytotoxic effect on HUVECs, but did not affect tube formation or the migration of endothelial cells. In addition, treatment of the xenograft mice with [Pt(DMS)] decreased VEGF, MMP1 and MMP2 expressions in tumours. CONCLUSIONS AND IMPLICATIONS The antiangiogenic and antitumour activities of [Pt(DMS)] provide a solid starting point for its validation as a suitable candidate for further pharmacological testing.

Published
2015

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