1. Qualitative evaluation of ferritin in serum samples by Raman spectroscopy and principal component analysis
- Author
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Teodoro Cordova-Fraga, Guadalupe de la Rosa-Alvarez, Juan Carlos Martínez-Espinosa, Rafael Guzmán-Cabrera, Blanca Murillo-Ortiz, J. Bernal-Alvarado, and Juan Manuel Ruvalcaba-López
- Subjects
Anemia ,Iron ,Dermatology ,Spectrum Analysis, Raman ,030207 dermatology & venereal diseases ,03 medical and health sciences ,symbols.namesake ,0302 clinical medicine ,Blood serum ,medicine ,Humans ,Principal Component Analysis ,Chromatography ,biology ,Chemistry ,Oxygen transport ,Discriminant Analysis ,030206 dentistry ,Serum samples ,medicine.disease ,Ferritin ,ROC Curve ,Ferritins ,Principal component analysis ,symbols ,biology.protein ,Surgery ,Hemoglobin ,Raman spectroscopy - Abstract
Iron molecule is of great importance in the synthesis of hemoglobin which is essential for oxygen transport. Iron levels are quantified by accurately high sensitivity tests, such as serum ferritin (SF). However, common studies to quantify SF are long and strenuous (~ 5 h), for example enzyme-linked immunosorbent assay (ELISA). In this paper, blood serum samples were analyzed by Raman spectroscopy (RS), and a computational analysis of spectra is proposed to detect differences in SF as an alternative procedure. Serum samples were obtained from 22 patients, 9 who were clinically diagnosed with anemia and 13 controls. Patients with anemia had low levels of SF (
- Published
- 2018