1. Transfer of mouse blastocysts exposed to ambient oxygen levels can lead to impaired lung development and redox balance
- Author
-
Nedim Karagenc, Mustafa Sandikçi, Hasan Yesilkaya, Göksel Doğan, Bengi Çinar Kul, Levent Karagenc, Hümeyra Ünsal, Kerem Esmen, Mehmet Orman, and Ege Üniversitesi
- Subjects
0301 basic medicine ,Embryology ,analytical parameters ,Organogenesis ,medicine.disease_cause ,Embryo Culture Techniques ,Mice ,chemistry.chemical_compound ,Bagg albino mouse ,0302 clinical medicine ,Pregnancy ,Gene expression ,oxidative stress ,glutathione ,gestational age ,Lung ,Mice, Inbred BALB C ,Fetal Growth Retardation ,030219 obstetrics & reproductive medicine ,quantitative analysis ,catalase ,malonaldehyde ,Obstetrics and Gynecology ,Embryo ,gene expression regulation ,intrauterine growth retardation ,cohort analysis ,Malondialdehyde ,superoxide dismutase ,unclassified drug ,3. Good health ,embryo culture ,fetus ,female ,oxidation reduction state ,medicine.anatomical_structure ,priority journal ,real time polymerase chain reaction ,validation study ,Female ,total antioxidant capacity ,Lung morphogenesis ,Oxidation-Reduction ,down regulation ,in vitro study ,immunoregulation ,phenotype ,animal experiment ,Embryonic Development ,embryo ,morphogenesis ,Fertilization in Vitro ,Biology ,Article ,animal tissue ,in vivo study ,Andrology ,03 medical and health sciences ,male ,Genetics ,medicine ,Animals ,C57BL 6 mouse ,controlled study ,Blastocyst ,atmospheric oxygen ,Molecular Biology ,cell transfer ,mouse ,lung development ,Fetus ,nonhuman ,blastocyst ,animal model ,assisted reproduction ,embryo development ,Embryo culture ,Cell Biology ,fetus weight ,clinical feature ,Mice, Inbred C57BL ,030104 developmental biology ,Reproductive Medicine ,chemistry ,exposure ,oxygen ,Oxidative stress ,Developmental Biology - Abstract
DOGAN, GOKSEL/0000-0002-4583-3140; CINAR KUL, Bengi/0000-0002-8955-0097, WOS: 000510017700006, PubMed: 31504752, In vitro culture under atmospheric oxygen puts embryos under oxidative stress and impairs preimplantation development. However, to what extent this process alters the redox balance in the perinatal period remains largely unknown. the aim of the present study was to examine if the redox balance is altered in the lung tissue of fetuses generated through transfer of mouse embryos exposed to atmospheric oxygen at different stages of development and to determine if this has any effect on lung morphogenesis and gene expression. Two experimental groups (EGs) were generated by transferring in vitro- and in vivo-derived blastocysts to pseudo-pregnant females. in vivo-developed fetuses served as control. Enzymatic/nonenzymatic antioxidants, malondialdehyde (MDA) levels, total antioxidant capacity, stage of lung development and gene expression were evaluated on day 18 of pregnancy. Weight of fetuses was significantly less in both experimental cohorts (ANOVA, P < 0.001 versus control), associated with delayed lung development, higher amounts of MDA (ANOVA, P < 0.001 versus control) and altered expression of several genes in oxidative stress/damage pathways. Evidence gathered in the present study indicates that pre-implantation stress caused by culture under atmospheric oxygen, even for a short period of time, leads to fetal growth restriction, impaired lung development and redox balance along with dysregulation of several genes in oxidative stress response. Absence of an EG in which in vitro embryo culture was performed at 5% oxygen and the use of genetically heterogeneous F2 fetuses are the limitations of the study. in any case, the long-term impact of such dramatic changes in the developmental programming of resulting fetuses warrants further investigations., Scientific and Technological Research Council of Turkey (TUB.ITAK)Turkiye Bilimsel ve Teknolojik Arastirma Kurumu (TUBITAK) [112O259], This work was funded by the Scientific and Technological Research Council of Turkey (TUB.ITAK, Project no: 112O259).
- Published
- 2019