1. Effect of andrographolide on cysteamine-induced duodenal ulcer in rats.
- Author
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Panneerselvam S, Arumugam G, and Karthikeyan NS
- Subjects
- Alkaline Phosphatase analysis, Animals, Cysteamine administration & dosage, Cysteamine adverse effects, Disease Models, Animal, Duodenal Ulcer chemically induced, Duodenal Ulcer enzymology, Duodenum drug effects, Duodenum enzymology, Glutathione analysis, Intestinal Mucosa drug effects, Intestinal Mucosa enzymology, Male, Microvilli drug effects, Microvilli enzymology, Plant Leaves chemistry, Rats, Rats, Wistar, Sucrase analysis, alpha-Glucosidases analysis, Andrographis chemistry, Anti-Ulcer Agents therapeutic use, Diterpenes administration & dosage, Diterpenes therapeutic use, Duodenal Ulcer drug therapy, Plant Extracts therapeutic use
- Abstract
The aim of this study was to evaluate the gastroprotective efficacy of andrographolide isolated from Andrographis paniculata in rats induced with duodenal ulcers. Duodenal ulcers were induced by cysteamine administration in rats pretreated with 3 mg kg⁻¹ BW day⁻¹ of andrographolide for 30 days. Ulcer score, myeloperoxidase activity, TBARS level, GSH/GSSG ratio and enzyme antioxidants were measured in the duodenal tissue. Brush border and basolateral membranes were isolated to assay sucrase, maltase, alkaline phosphatase and total ATPases. Ulcer score was significantly minimised in rats pretreated with andrographolide. Elevation in myeloperoxidase and TBARS levels were found to be minimised significantly due to andrographolide treatment. Membrane-bound enzyme activities and the thiol redox status of glutathione were significantly maintained in duodenal mucosa of rats that received andrographolide. This study reveals that the major component of A. paniculata, andrographolide, has potent antiulcer properties that are most likely caused by minimising inflammatory changes, counteracting free radical formation and maintaining the thiol redox status in the duodenum.
- Published
- 2011
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