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A widespread concern among ethnobiologists is the rapid process of erosion of indigenous environmental knowledge observed worldwide. This paper examines the ongoing transformations of knowledge about natural remedies in the Quechua-speaking Andes. Freelisting exercises and interviews were conducted with 36 households at Bolivian and Peruvian study sites. (Generalized) linear mixed-effects models were used to analyze the effects of age on knowledge about medicinal plants, animals, minerals, and their uses. Our study demonstrates that younger participants knew as much about natural remedies as their elders. However, proportional knowledge about some medicinal use categories of natural remedies varied with age. We conclude that knowledge about natural remedies is generally not being lost at the study sites. Nevertheless, it is undergoing transformations in terms of specific medicinal uses. A careful understanding of these complex transformation processes is needed to better orient initiatives for the conservation of biocultural diversity in the Andes and elsewhere. [ABSTRACT FROM AUTHOR]