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Negundoside and agnuside are reported as marker constituents for standardization of Vitex negundo (V. negundo) leaves. This is the first report on determination and quantification of negundoside and agnuside by qNMR in the leaves of V. negundo, collected from various regions of India. The developed qNMR method showed good linearity (r2 ≥ 0.9994), high precision (RSD < 5%) and a good recovery (99.79 and 101.35%) for both the compounds. Therefore, the developed qNMR method is accurate and reliable for the quality control of V. negundo. The qNMR method is useful in quality control of plant extracts without the need of extensive sample preparation and chromatographic separation.

Introduction Boerhaavia diffusa is a perennial herb belonging to Nyctaginaceae. Various classes of chemical constituents such as phenolics (boeravinones), terpenoids and organic acids have been reported in B. diffusa roots. As boeravinones have been proposed as putative active constituents for the anti-cancer, spasmolytic and anti-inflammatory activities exhibited by B. diffusa extracts, it is worthwhile developing and validating an ultra-performance liquid chromatography (UPLC) method for analysis of boeravinones in B. diffusa roots. Objective To develop and validate a simple, accurate, robust and rapid UPLC analytical method for quality control of B. diffusa roots. Methods Samples for analysis were prepared by refluxing powdered root material with methanol for 2 h. The extracts were concentrated, dried and stored at –20°C until their use. A UPLC with photodiode array (PDA) method was developed and validated for the quantification of boeravinones in the roots of B. diffusa. The separation of boeravinones was achieved using a BEH Shield C18-column (2.1 × 100 mm, 1.7 µm) with gradient elution of methanol and water (0.1% acetic acid), at a flow rate of 0.4 mL/min and detection was carried out at λmax 273 nm. Results The UPLC method developed showed good linearity (r2 ≥ 0.9999), accuracy and precision. Conclusion The UPLC method developed provided a selective, sensitive and rapid analytical method for the quantification of boeravinones in B. diffusa roots. All the validation parameters were found to be within the permissible limits as per International Conference on Harmonisation guidelines. Copyright © 2014 John Wiley & Sons, Ltd.

SFRE 199-1 medium (SFRE-M) is important mammalian cell culture medium, used for the culture of primary cells of mammals such as baboon kidney cells. The present study was attempted to evaluate the impact of biofield energy treatment on the physical, thermal and spectral properties of SFRE-M. The study was accomplished in two groups; one was set as control while another was subjected to Mr. Trivedi’s biofield energy treatment and coded as treated group. Subsequently, the control and treated samples were analyzed using various analytical techniques. The CHNO analysis showed about 2.16, 4.87, and 5.89% decrease in percent contents of carbon, hydrogen, and oxygen, respectively; while 9.49% increase in nitrogen contents of treated sample as compared to the control. X-ray diffraction (XRD) analysis showed 7.23% decrease in crystallite size of treated sample as compared to the control. The thermogravimetric analysis (TGA) analysis showed the increase in onset temperature of thermal degradation by 19.61% in treated sample with respect to the control. The control sample showed the 48.63% weight loss during the thermal degradation temperature (Tmax) while the treated sample showed only 13.62% weight loss during the Tmax. The differential scanning calorimetry (DSC) analysis showed the 62.58% increase in the latent heat of fusion of treated sample with respect to the control sample. The Fourier transform infrared spectroscopy (FT-IR) spectrum of treated SFRE-M showed the alteration in the wavenumber of C-O, C-N and C-H vibrations in the treated sample as compared to the control. Altogether, the XRD, TGA-DTG, DSC, and FT-IR analysis suggest that Mr. Trivedi’s biofield energy treatment has the impact on physical, thermal and spectral properties of SFRE-M. The treated SFRE-M was more thermal stable than the control SFRE-M and can be used as the better culture media for mammalian cell culture. https://www.trivedieffect.com/science/effect-of-biofield-treatment-on-physical-thermal-and-spectral-properties-of-sfre-199-1-mammalian-cell-culture-medium http://www.sciencepublishinggroup.com/journal/paperinfo?journalid=110&doi=10.11648/j.ab.20150306.13, Mahendra Kumar Trivedi, Alice Branton, Dahryn Trivedi, Gopal Nayak, Khemraj Bairwa, Snehasis Jana. Effect of Biofield Treatment on Physical, Thermal, and Spectral Properties of SFRE 199-1 Mammalian Cell Culture Medium. Advances in Biochemistry. Vol. 3, No. 6, 2015, pp. 77-85. doi: 10.11648/j.ab.20150306.13

The methylsulfonylmethane (MSM) is an organosulfur compound having sulfonyl functional group. It is occurred naturally in some primitive plants and used in disease related to chronic pain, inflammation, and arthritis. This study was attempted to evaluate the impact of biofield energy treatment on the physical, thermal, and spectral properties of MSM. The study was performed in two groups viz. the control group was remained as untreated, while the treated group was subjected to Mr. Trivedi’s biofield energy treatment. After that, both the control and treated samples were analyzed using surface area analyzer, X-ray diffraction (XRD), thermogravimetric analysis-derivative thermogravimetry (TGA-DTG), differential scanning calorimetry (DSC), and Fourier transform infrared (FT-IR) spectroscopy. The surface area analysis exhibited a significant decrease in the surface area of treated sample by 22.96% as compared to the control. The XRD analysis showed the significant increase in average crystallite size by 49.20% in the treated sample with respect to the control. The DSC analysis showed the significant increase (67.20%) in latent heat of fusion of treated sample with respect to the control. The TGA analysis showed the onset temperature of thermal degradation at 170°C in the control sample that was slightly decreased to 168.05°C after biofield treatment. Moreover, the Tmax (maximum thermal degradation temperature) was also decreased slightly from 186.66°C (control) to 183.38°C (treated). This indicated the early phase of vaporization in treated sample as compared to the control. The FT-IR spectroscopic study exhibited the alteration in wavenumber of S=O group that suggests the effect of biofield treatment on force constant and bond strength of MSM molecules. Altogether, the surface area, XRD, thermal analysis and FT-IR spectroscopy suggests that Mr. Trivedi’s biofield energy treatment has the impact on physical, thermal, and spectral properties of MSM. https://www.trivedieffect.com/science/evaluation-of-physical-thermal-and-spectral-parameters-of-biofield-energy-treated-methylsulfonylmethane https://www.omicsonline.org/open-access/evaluation-of-physical-thermal-and-spectral-parameters-of-biofieldenergy-treated-methylsulfonylmethane-2329-9053-1000129.php?aid=61799, Trivedi MK, Branton A, Trivedi D, Nayak G, Bairwa K, et al., (2015) Evaluation of Physical, Thermal and Spectral Parameters of Biofield Energy Treated Methylsulfonylmethane. J Mol Pharm Org Process Res 3:129. doi:10.4172/2329-9053.1000129

Triphenylmethane is a synthetic dye used as antimicrobial agent and for the chemical visualization in thin layer chromatography of higher fatty acids, fatty alcohols, and aliphatic amines. The present study was an attempt to investigate the impact of biofield treatment on physical, thermal and spectroscopical charecteristics of triphenylmethane. The study was performed in two groups i.e., control and treatment. The treatment group subjected to Mr. Trivedi’s biofield treatment. The control and treated groups of triphenylmethane samples were characterized using X-ray diffraction (XRD), surface area analyzer, differential scanning calorimetry (DSC), thermogravimetric analysis (TGA), Fourier transform infrared (FT-IR), ultraviolet-visible (UV-Vis) spectroscopy, and gas chromatography-mass spectrometry (GC-MS). XRD study revealed decreases in average crystallite size (14.22%) of treated triphenylmethane as compared to control sample. Surface area analysis showed a slight increase (0.42%) in surface area of treated sample with respect to control. DSC thermogram of treated triphenylmethane showed the slight increase in melting point and latent heat of fusion with respect to control. TGA analysis of control triphenylmethane showed weight loss by 45.99% and treated sample showed weight loss by 64.40%. The Tmax was also decreased by 7.17% in treated sample as compared to control. The FT-IR and UV spectroscopic result showed the similar pattern of spectra. The GC-MS analysis suggested a significant decrease in carbon isotopic abundance (expressed in δ13C, ‰) in treated sample (about 380 to 524‰) as compared to control. Based on these results, it is found that biofield treatment has the impact on physical, thermal and carbon isotopic abundance of treated triphenylmethane with respect to control. https://www.trivedieffect.com/science/physical-thermal-and-spectroscopical-characterization-of-biofield-treated-triphenylmethane-an-impact-of-biofield-treatment https://www.omicsonline.org/open-access/physical-thermal-and-spectroscopical-characterization-of-biofieldtreated-triphenylmethane-an-impact-of-biofield-treatment-2157-7064-1000292.php?aid=61490, Trivedi MK, Branton A, Trivedi D, Nayak G, Bairwa K, et al. (2015) Physical, Thermal and Spectroscopical Characterization of Biofield Treated Triphenylmethane: An Impact of Biofield Treatment. Method Validation and Estimation of the Uncertainty. J Chromatogr Sep Tech 6: 292.doi:10.4172/2157-7064.1000292

Disodium hydrogen orthophosphate is a water soluble white powder widely used as pH regulator and saline laxative. The sodium nitrate is a highly water soluble white solid, used in high blood pressure, dentinal hypersensitivity, and production of fertilizers. The present study was aimed to investigate the impact of biofield treatment on spectral properties of disodium hydrogen orthophosphate and sodium nitrate. The study was performed in two groups i.e., control and treatment of each compound. The treatment groups were subjected to Mr. Trivedi’s biofield treatment. The spectral properties of control and treated groups of both compounds were studied using Fourier transform infrared (FT-IR) and Ultraviolet-Visible (UV-Vis) spectroscopic techniques. FT-IR spectrum of biofield treated disodium hydrogen orthophosphate showed the shifting in wavenumber of vibrational peaks (with respect to control) corresponding to O-H stretching from 2975 to 3357 cm-1, PO-H symmetrical stretching from 2359 to 2350 cm-1, O=P-OH deformation from 1717-1796 cm-1 to 1701-1735 cm-1, P=O asymmetric stretching from 1356 to 1260 cm-1 and P=O symmetric stretching from 1159 to 1132 cm-1, etc. Likewise, the FT-IR spectrum of sodium nitrate exhibited the shifting of vibrational frequency of N=O stretching from 1788 to 1648 cm-1 and NO3 asymmetric and symmetric stretchings from 1369 to 1381 cm-1 and 1340 to 1267 cm-1. UV spectrum of treated disodium hydrogen orthophosphate revealed a negative absorbance; it may be due to decrease in UV absorbance as compared to control. UV spectrum of control sodium nitrate exhibited two absorbance maxima (λmax) at 239.4 nm and 341.4 nm, which were altered to one absorbance maxima (λmax) at 209.2 nm after biofield treatment. Overall, the FT-IR and UV spectroscopic data of both compounds suggest an impact of biofield treatment on spectral properties with respect to force constant, bond strength, dipole moments and transition energy between two orbitals (ground state and excited state) as compared to respective control. https://www.trivedieffect.com/science/spectroscopic-characterization-of-disodium-hydrogen-orthophosphate-and-sodium-nitrate-after-biofield-treatment https://www.omicsonline.org/open-access/spectroscopic-characterization-of-disodium-hydrogen-orthophosphate-and-sodium-nitrate-after-biofield-treatment-2157-7064-1000282.php?aid=59624+, Trivedi MK, Branton A, Trivedi D, Nayak G, Bairwa K, et al. (2015) Spectroscopic Characterization of Disodium Hydrogen Orthophosphate and Sodium Nitrate after Biofield Treatment. J Chromatogr Sep Tech 6: 282. doi:10.4172/2157-7064.1000282

p-Chlorobenzaldehyde (p-CBA) is used as an important chemical intermediate for the preparation of pharmaceuticals, agricultural chemicals, dyestuffs, optical brighteners, and metal finishing products. The study aimed to evaluate the effect of biofield energy treatment on the physicochemical and spectroscopic properties of p-CBA. The study was accomplished in two groups i.e. control and treated. The control group was remained as untreated, while the treated group was subjected to Mr. Trivedi’s biofield energy treatment. Finally, both the samples (control and treated) were evaluated using various analytical techniques. The surface area analysis showed a substantial increase in the surface area by 23.06% after biofield treatment with respect to the control sample. The XRD analysis showed the crystalline nature of both control and treated samples. The X-ray diffractogram showed the significant alteration in the peak intensity in treated sample as compared to the control. The XRD analysis showed the slight increase (2.31%) in the crystallite size of treated sample as compared to the control. The TGA analysis exhibited the decrease (10%) in onset temperature of thermal degradation form 140°C (control) to 126°C in treated sample. The Tmax (maximum thermal degradation temperature) was slightly decreased (2.14%) from 157.09°C (control) to 153.73°C in treated sample of p-CBA. This decrease in Tmax was possibly due to early phase of vaporization in treated sample as compared to the control. The FT-IR spectrum of treated p-CBA showed the increase in wavenumber of C=C stretching as compared to the control. The UV spectroscopic study showed the similar pattern of wavelength in control and treated samples. Altogether, the surface area, XRD, TGA-DTG and FT-IR analysis suggest that Mr. Trivedi’s biofield energy treatment has the impact to alter the physicochemical properties of p-CBA. This treated p-CBA could be utilized as a better chemical intermediate than the control p-CBA for the synthesis of pharmaceutical drugs and organic chemicals. https://www.trivedieffect.com/science/physicochemical-and-spectroscopic-characterization-of-p-chlorobenzaldehyde-an-impact-of-biofield-energy-treatment http://electroanalytical.imedpub.com/physicochemical-and-spectroscopiccharacterization-of-pchlorobenzaldehyde-animpact-of-biofield-energy-treatment.php?aid=7349, Trivedi MK, Branton A, Trivedi D, et al. Physicochemical and Spectroscopic Characterization of p-Chlorobenzaldehyde: An Impact of Biofield Energy Treatment. Insights Anal Electrochem. 2015, 1:1

Disulfiram is being used clinically as an aid in chronic alcoholism, while nicotinic acid is one of a B-complex vitamin that has cholesterol lowering activity. The aim of present study was to investigate the impact of biofield treatment on spectral properties of disulfiram and nicotinic acid. The study was performed in two groups i.e., control and treatment of each drug. The treatment groups were received Mr. Trivedi’s biofield treatment. Subsequently, spectral properties of control and treated groups of both drugs were studied using Fourier transform infrared (FT-IR) and Ultraviolet-Visible (UV-Vis) spectroscopic techniques. FT-IR spectrum of biofield treated disulfiram showed the shifting in wavenumber of C-H stretching from 1496 to 1506 cm-1 and C-N stretching from 1062 to 1056 cm-1. The intensity of S-S dihedral bending peaks (665 and 553 cm-1) was also increased in biofield treated disulfiram sample, as compared to control. FT-IR spectra of biofield treated nicotinic acid showed the shifting in wavenumber of C-H stretching from 3071 to 3081 cm-1 and 2808 to 2818 cm-1. Likewise, C=C stretching peak was shifted to higher frequency region from 1696 cm-1 to 1703 cm-1 and C-O (COO-) stretching peak was shifted to lower frequency region from 1186 to 1180 cm-1 in treated nicotinic acid. UV spectrum of control and biofield treated disulfiram showed similar pattern of UV spectra. Whereas, the UV spectrum of biofield treated nicotinic acid exhibited the shifting of absorption maxima (λmax) with respect of control i.e., from 268.4 to 262.0 nm, 262.5 to 256.4, 257.5 to 245.6, and 212.0 to 222.4 nm. Over all, the FT-IR and UV spectroscopy results suggest an impact of biofield treatment on the force constant, bond strength, and dipole moments of treated drugs such as disulfiram and nicotinic acid that could led to change in their chemical stability as compared to control. https://www.trivedieffect.com/science/spectroscopic-characterization-of-disulfiram-and-nicotinic-acid-after-biofield-treatment https://www.omicsonline.org/open-access/spectroscopic-characterization-of-disulfiram-and-nicotinic-acid-after-biofieldtreatment-2155-9872-1000265.php?aid=60834, Trivedi MK, Branton A, Trivedi D, Nayak G, Bairwa K, et al. (2015) Spectroscopic Characterization of Disulfiram and Nicotinic Acid after Biofield Treatment. J Anal Bioanal Tech 6: 265 doi:10.4172/2155-9872.1000265

Ammonium acetate and ammonium chloride are the white crystalline solid inorganic compounds having wide application in synthesis and analytical chemistry. The aim of present study was to evaluate the impact of biofield treatment on spectral properties of inorganic salt like ammonium acetate and ammonium chloride. The study was performed in two groups of each compound i.e., control and treatment. Treatment groups were received Mr. Trivedi’s biofield treatment. Subsequently, control and treated groups were evaluated using Fourier Transform Infrared (FT-IR) and Ultraviolet-Visible (UV-Vis) spectroscopy. FT-IR spectrum of treated ammonium acetate showed the shifting in wavenumber of vibrational peaks with respect to control. Like, the N-H stretching was shifted from 3024-3586 cm-1 to 3033-3606 cm-1, C-H stretching from 2826-2893 cm-1 to 2817-2881 cm-1, C=O asymmetrical stretching from 1660-1702 cm-1 to 1680-1714 cm-1, N-H bending from 1533-1563 cm-1 to 1506-1556 cm-1 etc. Treated ammonium chloride showed the shifting in IR frequency of three distinct oscillation modes in NH4 ion i.e., at ν1, 3010 cm-1 to 3029 cm-1; ν2, 1724 cm-1 to 1741 cm-1; and ν3, 3156 cm-1 to 3124 cm-1. The N-Cl stretching was also shifted to downstream region i.e., from 710 cm-1 to 665 cm-1 in treated ammonium chloride. UV spectrum of treated ammonium acetate showed the absorbance maxima (λmax) at 258.0 nm that was shifted to 221.4 nm in treated sample. UV spectrum of control ammonium chloride exhibited two absorbance maxima (λmax) i.e., at 234.6 and 292.6 nm, which were shifted to 224.1 and 302.8 nm, respectively in treated sample. Overall, FT-IR and UV data of both compounds suggest an impact of biofield treatment on atomic level i.e., at force constant, bond strength, dipole moments and electron transition energy between two orbitals of treated compounds as compared to respective control. https://www.trivedieffect.com/science/fourier-transform-infrared-and-ultraviolet-visible-spectroscopic-characterization-of-ammonium-acetate-and-ammonium-chloride-an-impact-of-biofield-treatment https://www.omicsonline.org/open-access/fourier-transform-infrared-and-ultravioletvisible-spectroscopic-characterization-of-ammonium-acetate-and-ammonium-chloride-animpact-of-biofield-treatment-2329-6798-1000163.php?aid=58879+, Trivedi MK, Branton A, Trivedi D, Nayak G, Bairwa K, et al. (2015) Fourier Transform Infrared and Ultraviolet-Visible Spectroscopic Characterization of Ammonium Acetate and Ammonium Chloride: An Impact of Biofield Treatment. Mod Chem appl 3:163. doi:10.4172/2329-6798.1000163

The p-anisidine is widely used as chemical intermediate in the production of various dyes, pigments, and pharmaceuticals. This study was aimed to evaluate the effect of biofield energy treatment on the physicochemical and spectroscopic properties of p-anisidine. The study was performed after dividing the sample in two groups; one was remained as untreated and another was subjected to Mr. Trivedi’s biofield energy treatment. Afterward, both the control and treated samples of p-anisidine were evaluated using X-ray diffraction (XRD), surface area analyzer, differential scanning calorimetry (DSC), thermogravimetric analysis-derivative thermogravimetry (TGA-DTG), Fourier transform infrared (FT-IR), and ultraviolet-visible (UV-Vis) spectroscopy. The XRD analysis showed the increase in unit cell volume from 683.81 → 690.18 × 10-24 cm3 and crystallite size from 83.84→84.62 nm in the treated sample with respect to the control. The surface area analysis exhibited the significant increase (25.44%) in the surface area of treated sample as compared to control. The DSC thermogram of control p-anisidine showed the latent heat of fusion and melting temperature and 146.78 J/g and 59.41°C, respectively, which were slightly increased to 148.89 J/g and 59.49°C, respectively after biofield treatment. The TGA analysis showed the onset temperature of thermal degradation at 134.68°C in the control sample that was increased to 150.02°C after biofield treatment. The result showed about 11.39% increase in onset temperature of thermal degradation of treated p-anisidine as compared to the control. Moreover, the Tmax (temperature at which maximum thermal degradation occurs) was also increased slightly from 165.99°C (control) to 168.10°C (treated). This indicated the high thermal stability of treated p-anisidine as compared to the control. However, the FT-IR and UV spectroscopic studies did not show any significant changes in the spectral properties of treated p-anisidine with respect to the control. All together, the XRD, surface area and thermal analysis suggest that Mr. Trivedi’s biofield energy treatment has the impact on physical and thermal properties of the treated p-anisidine. https://www.trivedieffect.com/science/physicochemical-and-spectroscopic-characterization-of-biofield-energy-treated-p-anisidine https://www.omicsonline.org/open-access/physicochemical-and-spectroscopic-characterization-of-biofield-energytreated-panisidine-paco-1000102.php?aid=60583, Trivedi MK, Branton A, Trivedi D, Nayak G, Bairwa K, et al (2015) Physicochemical and Spectroscopic Characterization of Biofield Energy Treated p-Anisidine. Pharm Anal Chem Open Access 1: 102. doi:10.4172/2471- 2698.1000102

Salicylic acid is a naturally occurring derivative of benzoic acid, and widely used in organic synthesis and as a plant hormone. Sparfloxacin is fluorinated quinolone antibiotic having broad spectrum antimicrobial property. The present study was aimed to evaluate the impact of biofield treatment on spectral properties of salicylic acid and sparfloxacin using FT-IR and UV-Vis spectroscopic techniques. The study was carried out in two groups, one was set to control, and another was subjected to biofield treatment. FT-IR spectrum of treated salicylic acid showed the upstream shifting in wavenumber of C-H stretching from 2999 to 3004 cm-1 and 2831 to 2837 cm-1 and C=O asymmetric stretching vibration from 1670 to 1683 cm-1 and 1652 to 1662 cm-1. The peak intensity in treated salicylic acid at 1558 cm-1 (aromatic C=C stretching) and 1501 cm-1 (C-C stretching) was increased as compared to control. FT-IR spectrum of treated sparfloxacin showed a downstream shifting in wavenumber of C-H stretching from 2961 to 2955 cm-1 and 2848 to 2818 cm-1, and upstream shifting in wavenumber of C=O (pyridone) stretching from 1641 to 1648 cm-1. Besides, increased intensity of peaks in treated sparfloxacin was found at 1628 cm-1 [C=C stretching (pyridone)] and 1507 cm-1 (N-H bending) as compared to control. UV spectrum of biofield treated salicylic acid exhibited a shifting of wavelength (λmax) from 295.8 to 302.4 nm and 231.2 to 234.4 nm, with respect to control. Likewise, biofield treated sparfloxacin showed the shifting in UV wavelength (λmax) from 373.8 to 380.6 nm and 224.2 to 209.2 nm. Over all, the results suggest that alteration in wavenumber of IR peaks in treated samples might be occurred due to biofield induced alteration in force constant and dipole moment of some bonds. The changes in UV wavelength (λmax) of treated sample also support the FT-IR results. Due to alteration in force constant and bond strength, the chemical stability of structure of treated drugs might also be increased, which could be beneficial for self-life of biofield treated drugs. Source: https://www.trivedieffect.com/science/fourier-transform-infrared-and-ultraviolet-visible-spectroscopic-characterization-of-biofield-treated-salicylic-acid-and-sparfloxacin https://www.omicsonline.org/open-access/fourier-transform-infrared-and-ultravioletvisible-spectroscopiccharacterization-of-biofield-treated-salicylic-acid-and-sparfloxacin-2329-6836-1000186.php?aid=59883+

Five new (2, 3, 5, 7, and 9) and four known rotenoids (1, 4, 6, and 8) were isolated from a methanol extract of Boerhaavia diffusa roots. The structures of the new rotenoids were elucidated by spectroscopic data interpretation. The 70% ethanol extract, a rotenoid-rich fraction, and all isolated rotenoids were evaluated for their COX-1 and COX-2 inhibitory activities. Among the rotenoids tested, compound 7 showed the most potent COX-1 and COX-2 inhibition, with IC₅₀ values of 21.7 ± 0.5 and 25.5 ± 0.6 μM, respectively. Boeravinone B (6) exhibited significant anti-inflammatory activity (56.6% at 50 mg/kg) when evaluated in an in vivo carrageenan-induced rat paw model.

p-Chlorobenzophenone (p-CBP) is the important chemical intermediate used for the synthesis of several pharmaceutical drugs like fenofibrate, cetirizine, alprazolam, and benzodiazepine. The aim of this study was set to evaluate the impact of biofield energy treatment on physicochemical and spectroscopic properties of p-CBP. The study was accomplished in two groups i.e. control and treated. The treated group was subjected to Mr. Trivedi’s biofield energy treatment. Subsequently, the control and treated samples of p-CBP were analyzed using X-ray diffraction (XRD), particle size analyzer, surface area analyzer, differential scanning calorimetry (DSC), thermogravimetric analysis-derivative thermogravimetric analysis (TGA-DTG), Fourier transform infrared (FT-IR), and ultraviolet-visible (UV-Vis) spectroscopy. The XRD study exhibited the increase in average crystallite size (25.93%) as well as the intensity of XRD peaks of treated p-CBP, as compared to the control. The particle size analysis showed the reduction in particle size of fine particles (≤51.49 µm) by 21.6% (d10), whereas, increase in particle size of large particles (≥433.59 µm) by 12.82% (d90) and 17.71% (d99), respectively after biofield treatment, as compared to the control. The surface area analysis exhibited the surface area as 0.7005 m2/g in control and 0.7020 m2/g in treated sample of p-CBP. The DSC thermogram of treated p-CBP exhibited the slight decrease in melting temperature. However, the latent heat of fusion was significantly altered (24.90%) after biofield energy treatment as compared to the control. TGA analysis showed the weight loss by 57.36% in control and 58.51% in treated sample. In addition, the onset temperature of thermal degradation was also decreased by 6.32% after biofield energy treatment as compared to the control p-CBP. The FT-IR and UV spectroscopic study did not show the alteration in the wavenumber and wavelength, respectively in treated p-CBP as compared to the control. Altogether, the XRD, particle size and thermal analysis suggest that biofield energy treatment has significant impact on physical and thermal properties of treated p-CBP.

Protose is the enzyme digest of mixed proteins that is recommended for culture media, bulk production of enzymes, antibiotics, toxins, veterinary preparations, etc. This study was proposed to evaluate the effect of biofield energy treatment on the physicochemical and spectroscopic properties of protose. The study was achieved in two groups i.e. control and treated. The control group was remained as untreated, while the treated group was received Mr. Trivedi’s biofield energy treatment. Finally, both the control and treated samples were evaluated using various analytical techniques. The X-ray diffractograms (XRD) of control and treated samples showed the halo patterns peak that suggested the amorphous nature of both the samples of protose. The particle size analysis showed about 12.68% and 90.94 increase in the average particle size (d50) and d99 (particle size below which 99% particles are present) of treated protose with respect to the control. The surface area analysis revealed the 4.96% decrease in the surface area of treated sample as compared to the control sample. The differential scanning calorimetry (DSC) analysis revealed the 22.49% increase in the latent heat of fusion of treated sample as compared to the control. Thermogravimetric analysis (TGA) analysis showed increase in maximum thermal degradation temperature (Tmax) by 5.02% in treated sample as compared to the control. The increase in Tmax might be correlated with increased thermal stability of treated sample as compared to the control. Fourier transform infrared (FT-IR) study showed the alteration in the vibrational frequency of functional groups like N-H, C-H, and S=O of treated protose as compared to the control sample. Based on the overall analytical results, it is concluded that Mr. Trivedi’s biofield energy treatment has a significant impact on the physicochemical and spectral properties of protose. As a result, the treated protose might be more effective as a culture medium than the corresponding control. https://www.trivedieffect.com/science/physicochemical-and-spectroscopic-properties-of-biofield-energy-treated-protose http://www.sciencepublishinggroup.com/journal/paperinfo?journalid=655&doi=10.11648/j.ajbls.20150306.11, Mahendra Kumar Trivedi, Alice Branton, Dahryn Trivedi, Gopal Nayak, Khemraj Bairwa, Snehasis Jana. Physicochemical and Spectroscopic Properties of Biofield Energy Treated Protose. American Journal of Biomedical and Life Sciences. Vol. 3, No. 6, 2015, pp. 104-110. doi: 10.11648/j.ajbls.20150306.11