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Your search keyword '"B. C. Goverde"' showing total 3 results
Start Over Author "B. C. Goverde" Topic analytical chemistry
3 results on '"B. C. Goverde"'

1. HPLC analysis of phenylthiocarbamyl (PTC) amino acids. I. Evaluation and optimization of the procedure

Author

H. W. M. van den Bogaart, B. C. Goverde, J. W. Van Nispen, R. L. A. E. Hamelinck, P. S. L. Janssen, and P. A. T. A. Melgers

Subjects
chemistry.chemical_classification, Hplc analysis, Chromatography, endocrine system diseases, Organic Chemistry, Clinical Biochemistry, Peptide, Buffer solution, Biochemistry, High-performance liquid chromatography, Analytical Chemistry, Amino acid, chemistry.chemical_compound, Hydrolysis, Amino acid analysis, chemistry, Derivatization
Abstract

An HPLC system has been developed in which 23 phenylthiocarbamyl (PTC) amino acids are distinctly separated. Applying this system, the preceding steps in HPLC amino acid analysis i.e. gas-phase hydrolysis of the protein/peptide sample and the pre-column derivatization, were evaluated and optimized. PTC amino acids in HPLC buffer solution are stable for at least 16 hours if stored at 5°–8°C. The sensitivity of the method is in the low pmol range.

Published
1986
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2. HPLC analysis of phenylthiocarbamyl (PTC) amino acids II. Application in the analysis of (poly)peptides

Author

J. W. Van Nispen, H. W. M. van den Bogaart, P. A. T. A. Melgers, G. W. M. van Aalst, B. C. Goverde, and P. S. L. Janssen

Subjects
chemistry.chemical_classification, Hplc analysis, Chromatography, Organic Chemistry, Clinical Biochemistry, Peptide, Biochemistry, High-performance liquid chromatography, Analytical Chemistry, Gas phase, Amino acid, chemistry.chemical_compound, Hydrolysis, Amino acid analysis, chemistry, Derivatization
Abstract

The composition of 10 known (poly)peptides of widely different nature was determined by a previously optimized HPLC-PTC amino acid analysis; for the gasphase hydrolysis and the derivatization step use was made of a Pico-Tag Workstation. Results are in good agreement with those of the classical Moore and Stein technique.

Published
1986
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3. Application of reversed-phase HPLC in some critical peptide separations

Author

R. L. A. E. Hamelinck, B. C. Goverde, J. W. Van Nispen, P. A. T. A. Melgers, and P. S. L. Janssen

Subjects
Chemical Phenomena, Swine, Peptide, High-performance liquid chromatography, Analytical Chemistry, Adrenocorticotropic Hormone, Animals, Humans, Insulin, Endorphins, Enantiomeric excess, Chromatography, High Pressure Liquid, chemistry.chemical_classification, Chromatography, Chemistry, Chemistry, Physical, beta-Endorphin, INSULIN PREPARATIONS, General Medicine, Reversed-phase chromatography, Peptide Fragments, Rats, Peptide chemistry, Cattle, Peptides
Abstract

The application of reversed-phase high performance liquid chromatography in peptide chemistry is illustrated. Its use is described for separation of closely related endorphins, as an optical purity criterion, for rapid determination of the species of origin of insulin preparations, and finally for analysis of small, basic (hydrophilic) peptides.

Published
1984

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