1. Cumulative effects of repetitive intermittent hypercapnic hypoxia on orexin in the developing piglet hypothalamus
- Author
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Karen A. Waters, Man K. Du, Rita Machaalani, and Nicholas J. Hunt
- Subjects
0301 basic medicine ,medicine.medical_specialty ,Time Factors ,Swine ,Hypothalamus ,Neuropeptide ,Hypercapnia ,03 medical and health sciences ,0302 clinical medicine ,Developmental Neuroscience ,Orexin Receptors ,Internal medicine ,medicine ,Animals ,Receptor ,Hypoxia ,Analysis of Variance ,Orexins ,business.industry ,Gene Expression Regulation, Developmental ,Hypoxia (medical) ,Sudden infant death syndrome ,Orexin receptor ,Orexin ,body regions ,030104 developmental biology ,Endocrinology ,Animals, Newborn ,Models, Animal ,Wakefulness ,medicine.symptom ,business ,030217 neurology & neurosurgery ,Developmental Biology - Abstract
Orexin neuropeptides (OxA and OxB) and their receptors (OX1R and OX2R) are involved in maintenance of sleep and wakefulness, and are regulated by various environmental stimuli. We studied piglets, in the early neonatal period, exposed to 48-min of intermittent hypercapnic hypoxia (IHH; 7% O2/8% CO2) alternating with air. Three groups of 13-14 day-old piglets with IHH exposure of 1-day (1D-IHH) (n=7), 2-days (2D-IHH) (n=7) and 4-days (4D-IHH) (n=8) were compared to controls (exposed only to air, n=8). Immunoreactivity of OxA and OxB was studied in the piglet hypothalamic regions of the dorsomedial hypothalamus (DMH), perifornical area (PeF) and lateral hypothalamic area (LH). Results showed that after 1D- and 2D-IHH, total OxA and OxB expression decreased by 20% (p ≤ 0.005) and 40% (p
- Published
- 2015