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Nutritional disorders are problems in the poultry industry. This experiment aimed to study the effect of high doses of soy oil and lysine (Lys) on the liver and Pectoralis muscle (PM) in broiler during the starter period and apply I See Inside (ISI) and semiquantitative methodology, determined the correlation between pathological lesions in both organs and regression assessment for the effects of myopathies on the liver. Forty broilers were divided into T1, receiving basal diet T2 and T3, high additive oil and lysine 4.56 and 1.66% respectively, and T4, which received both high additives; the trial continued for 12 days. The total ISI index for histopathological lesions in the liver and PM was 74 and 41, respectively, represented by vascular, degeneration alteration, and necrosis; ISI assessment revealed highly significant lesions in the liver of all groups in comparison with T1, significance endomias tissue formation in T4 at 4.00, splitting PM fiber in both T3 and T4 1.60, the intermittent muscle fiber of the broiler at same treatments was 1.00, furthermore semi-quantitative analysis investigated significant correlation between infiltration of inflammatory cells with highly significant congestion correlation with necrosis in the liver. In contrast, in the muscle, the necrosis is significantly correlated with hemorrhage in the liver; the regression plot analysis also determined the R2 value for microscopic lesions in the muscle, which affected hepatic tissue. This study concludes that ISI with semi-quantitively analysis is a sound methodology for determining the significant deleterious effects of high doses of oil and lysine in broil ration.