1. Analgesic effect of the main components of Corydalis yanhusuo (yanhusuo in Chinese) is caused by inhibition of voltage gated sodium channels.
- Author
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Xu Y, Sun J, Li W, Zhang S, Yang L, Teng Y, Lv K, Liu Y, Su Y, Zhang J, and Zhao M
- Subjects
- Analgesics chemistry, Analgesics isolation & purification, Animals, Berberine Alkaloids isolation & purification, Berberine Alkaloids pharmacology, CHO Cells, Cricetulus, Disease Models, Animal, Drugs, Chinese Herbal chemistry, Drugs, Chinese Herbal pharmacology, Formaldehyde, Mice, Pain drug therapy, Patch-Clamp Techniques, Voltage-Gated Sodium Channel Blockers chemistry, Voltage-Gated Sodium Channel Blockers isolation & purification, Voltage-Gated Sodium Channels drug effects, Voltage-Gated Sodium Channels metabolism, Analgesics pharmacology, Corydalis chemistry, Drugs, Chinese Herbal poisoning, Voltage-Gated Sodium Channel Blockers pharmacology
- Abstract
Ethnopharmacology Relevance: Pain often causes a series of abnormal changes in physiology and psychology, which can lead to disease and even death. Drug therapy is the most basic and commonly used method for pain relief and management. Interestingly, at present, hundreds of traditional Chinese medicines have been reported to be used for pain relief, most of which are monomer preparations, which have been developed into new painkillers. Corydalis yanhusuo is a representative of one of these medicines and is available for pain relief., Aim of the Study: This study aims to determine the analgesic effect and the potential targets of the monomers derived from Corydalis yanhusuo, and to explore any possible associated cardiac risk factors., Materials and Methods: In this study, four monomers derived from Corydalis yanhusuo (tetrahydropalmatine, corydaline, protopine, dehydrocorydaline) were tested in vivo, using the formalin-induced pain model to determine their analgesic properties. Their potential targets were also determined using whole cell patch clamp recordings and myocardial enzyme assays., Results: The results showed that all monomers showed analgesic activity and inhibited the peak currents, promoted the activation and inactivation phases of Nav1.7, which indicating that Nav1.7 might be involved in the analgesic mechanism of Corydalis yanhusuo. Protopine increased the level of creatine kinase-MB (CK-MB) and inhibited the peak currents, promoted the activation and inactivation phases of Nav1.5, indicating that Nav1.5 might be involved in the cardiac risk associated with protopine treatment., Conclusion: These data showed that tetrahydropalmatine produced the best analgesic effect and the lowest cardiac risk. Thus, voltage gated sodium channels (VGSCs) might be the main targets associated with Corydalis yanhusuo. This study, therefore, provides valuable information for future studies and use of traditional Chines medicines for the alleviation of pain., (Copyright © 2021 Elsevier B.V. All rights reserved.)
- Published
- 2021
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