1. In Situ Synthesis of Silver Nanoparticles in a Hydrogel of Carboxymethyl Cellulose with Phthalated-Cashew Gum as a Promising Antibacterial and Healing Agent.
- Author
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Lustosa AKMF, de Jesus Oliveira AC, Quelemes PV, Plácido A, da Silva FV, Oliveira IS, de Almeida MP, Amorim ADGN, Delerue-Matos C, de Oliveira RCM, da Silva DA, Eaton P, and de Almeida Leite JRS
- Subjects
- Animals, Male, Metal Nanoparticles ultrastructure, Microbial Sensitivity Tests, Particle Size, Pseudomonas aeruginosa drug effects, Rats, Wistar, Rheology, Staphylococcus aureus drug effects, Anacardium chemistry, Anti-Bacterial Agents pharmacology, Carboxymethylcellulose Sodium chemistry, Hydrogel, Polyethylene Glycol Dimethacrylate chemistry, Metal Nanoparticles chemistry, Phthalic Acids chemistry, Silver pharmacology, Wound Healing drug effects
- Abstract
Silver nanoparticles have been shown to possess considerable antibacterial activity, but in vivo applications have been limited due to the inherent, but low, toxicity of silver. On the other hand, silver nanoparticles could provide cutaneous protection against infection, due to their ability to liberate silver ions via a slow release mechanism, and their broad-spectrum antimicrobial action. Thus, in this work, we describe the development of a carboxymethyl cellulose-based hydrogel containing silver nanoparticles. The nanoparticles were prepared in the hydrogel in situ, utilizing two variants of cashew gum as a capping agent, and sodium borohydride as the reducing agent. This gum is non-toxic and comes from a renewable natural source. The particles and gel were thoroughly characterized through using rheological measurements, UV-vis spectroscopy, nanoparticles tracking analysis, and transmission electron microscopy analysis (TEM). Antibacterial tests were carried out, confirming antimicrobial action of the silver nanoparticle-loaded gels. Furthermore, rat wound-healing models were used and demonstrated that the gels exhibited improved wound healing when compared to the base hydrogel as a control. Thus, these gels are proposed as excellent candidates for use as wound-healing treatments., Competing Interests: The authors declare no conflict of interest.
- Published
- 2017
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