1. Two methyl ester derivatives of microcystins, cyclic heptapeptide hepatotoxins, isolated from Anabaena flos-aquae strain CYA 83/1.
- Author
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Sivonen K, Skulberg OM, Namikoshi M, Evans WR, Carmichael WW, and Rinehart KL
- Subjects
- Amino Acid Sequence, Animals, Chemical and Drug Induced Liver Injury etiology, Chromatography, High Pressure Liquid, Female, Marine Toxins, Mice, Microcystins, Molecular Sequence Data, Peptides, Cyclic toxicity, Spectrometry, Mass, Fast Atom Bombardment, Anabaena chemistry, Peptides, Cyclic analysis
- Abstract
Cultured cells of Anabaena flos-aquae strain CYA 83/1, isolated from Lake Edlandsvatn, Norway, produced two microcystin mono-methyl ester derivatives (1 and 2) at the D-Glu unit in addition to microcystin-LR (3), [D-Asp3]microcystin-LR (4), microcystin-RR (5), and [D-Asp3]microcystin-RR (6). Structures of these compounds were assigned based on their amino acid analysis with a Waters Pico Tag HPLC system plus fast atom bombardment mass spectrometry (FABMS), including tandem FABMS, analysis on the two new microcystins, [D-Glu(OCH3)6]microcystin-LR (1) and [D-Asp3, D-Glu(OCH3)6]microcystin-LR (2). Toxicity data were not obtained for 1 and 2 because of the small amounts isolated from the cells.
- Published
- 1992
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