1. Enzyme identification of thermophilic bacteria from geothermal hotspring in Simbolon village, North Sumatera, Indonesia.
- Author
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Rosda, Dian Hardiyanti, Fachrial, Edy, Harmileni, Nugroho, Titania T., and Saryono
- Subjects
THERMOPHILIC bacteria ,ENZYMES ,BACILLUS amyloliquefaciens ,AMYLASES ,MANUFACTURING processes ,CELLULASE ,GELATIN ,DIGESTIVE enzymes - Abstract
Isolated Enzyme from thermophilic bacteria is useful in a wide variety of industries, including the manufacturing industry which relies on high temperatures. These enzymes provide several benefits since they may speed up reactions, reducing manufacturing costs, energy consumption, and time in the industrial process. Here we report UTMSBA and UTMSBS isolates that showed positive results in the gelatin hydrolysis test, while the citrate test, catalase test, sulfide hydrolysis, gelatin hydrolysis, and mortality test showed negative results. The edges and elevations of UTMSBA and UTMSBS isolates appear circular, entire, and flat. The isolates were able to hydrolyze amylase, cellulase, and protease enzymes, and the 16SrRNA gene revealed 99.99 percent homology, indicating Bacillus amyloliquefaciens strain V4. [ABSTRACT FROM AUTHOR]
- Published
- 2023
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