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1-Amino-2,2-difluorocyclopropane-1-carboxylicacid (DFACC)is of interest in the study of 1-aminocyclopropane-1-carboxylicacid (ACC) deaminase due to the increased reactivity of its cyclopropylfunctionality. It is shown that DFACC is unstable under near-physiologicalconditions where it primarily decomposes via specific-base catalysisto 3-fluoro-2-oxobut-3-enoic acid with a rate constant of 0.18 ±0.01 min–1. Upon incubation with ACC deaminase,DFACC is found to be a slow-dissociating inhibitor of ACC deaminasewith submicromolar affinity. [ABSTRACT FROM AUTHOR]