1. X-ray structure of the metal-sensor CnrX in both the apo- and copper-bound forms
- Author
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Pompidor, Guillaume, Maillard, Antoine P., Girard, Eric, Gambarelli, Serge, Kahn, Richard, and Covès, Jacques
- Subjects
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APOLIPOPROTEINS , *COPPER proteins , *CHEMICAL detectors , *RALSTONIA , *GENETIC transduction , *AMINO acids , *METALLOPROTEINS , *HEAVY metals - Abstract
Abstract: Both the X-ray structures of the apo- and the copper-bound forms of the metal-sensor domain (residues 31–148) of CnrX from Cupriavidus metallidurans CH34 were obtained at 1.74Å resolution from a selenomethionine derivative. This four-helix hooked-hairpin is the first structure of a metal-sensor in an ECF-type signaling pathway. The copper ion is bound in a type 2-like center with a 3N1O coordination in the equatorial plane and shows an unprecedented remote fifth axial ligand with Met93 contributing a weak S–Cu bond. The signal onset cannot be explained by conformational changes associated with CnrX metallation. [Copyright &y& Elsevier]
- Published
- 2008
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