1. Diamino acid derivatives of porphyrins penetrate into yeast cells, induce photodamage, but have no mutagenic effect.
- Author
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Zoładek T, Nguyen BN, Jagiełło I, Graczyk A, and Rytka J
- Subjects
- Amino Acids, Diamino pharmacology, Hematoporphyrins chemistry, Hematoporphyrins pharmacology, Mutagenesis, Photochemistry, Photosensitizing Agents chemistry, Photosensitizing Agents pharmacology, Protoporphyrins chemistry, Protoporphyrins pharmacology, Saccharomyces cerevisiae genetics, Amino Acids, Diamino metabolism, Hematoporphyrins metabolism, Photosensitizing Agents metabolism, Protoporphyrins metabolism, Saccharomyces cerevisiae metabolism
- Abstract
The yeast Saccharomyces cerevisiae was used as a model eukaryotic organism to study the uptake of diamino acid derivatives of porphyrins and their phototoxicity with particular emphasis on possible mutagenic effects. The water-soluble hematoporphyrin derivatives diarginate (HpD[Arg]2) and 1-arginin di(N-amino acid)-protoporphyrinate used in this study are effective photosensitizers in tumor photodynamic therapy. Depending on the amino acid substituent, the porphyrin derivatives differ in their affinity for yeast cells. It is shown that HpD(Arg)2 and PP(Met)2 (Arg)2 penetrate into the yeast cell and are metabolized. Both compounds sensitize yeast cells to photodamage but have no mutagenic effect on nuclear or mitochondrial genomes.
- Published
- 1997
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