1. Molecular cloning and characterization of an almond 9-hydroperoxide lyase, a new CYP74 targeted to lipid bodies
- Author
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Gian Pietro Di Sansebastiano, Angela Quarta, Rina Iannacone, Angelo De Paolis, Rod Casey, Angelo Santino, Giovanni Mita, Carla Perrotta, Pasqua Fasano, Richard K. Hughes, Eric J. Belfield, Nicolas Tsesmetzis, Mita, G., Quarta, A., Fasano, P., De Paolis, A., Di Sansebastiano, G. P., Perrotta, C., Iannacone, R., Belfield, E., Hughes, R., Tsesmetzis, N., Casey, R., and Santino, A.
- Subjects
Physiology ,Recombinant Fusion Proteins ,Molecular Sequence Data ,Oxylipin ,Plant Science ,Molecular cloning ,Almond ,Hydroperoxide lyase ,Gene expression ,Endomembrane system ,Amino Acid Sequence ,Seed development ,Cloning, Molecular ,Hydro-Lyases ,Phylogeny ,DNA Primers ,Lipid body ,chemistry.chemical_classification ,Genomic Library ,Base Sequence ,Sequence Homology, Amino Acid ,biology ,Reverse Transcriptase Polymerase Chain Reaction ,Cytochrome P450 ,food and beverages ,Lipid Metabolism ,Lyase ,Recombinant Proteins ,Enzyme assay ,Enzyme ,Biochemistry ,chemistry ,Seeds ,biology.protein ,Prunus ,Sequence Alignment ,Polyunsaturated fatty acid - Abstract
Oxylipin metabolism represents one of many defence mechanisms employed by plants. It begins with the oxygenation of polyunsaturated fatty acids by lipoxygenases to form fatty acid hydroperoxides that are substrates for several enzymes, including specialized cytochrome P450s known as CYP74s. The targeting of a new CYP74, a 9-hydroperoxide lyase (HPL) from almonds, to the endomembrane system and lipid bodies, both as enzyme activity in almond seeds and as GFP fusions transiently expressed in tobacco protoplasts, is described. Such association of a CYP74 with lipid bodies has not been reported previously. Also described are the properties of a 9-HPL gene, the developmental regulation of its expression, the production and characterization of recombinant 9-HPL. in Escherichia coli, and the developmental correlation between gene expression, enzyme activity, and the appearance of volatile C9 aldehydes from HPL action. © The Author [2005]. Published by Oxford University Press [on behalf of the Society for Experimental Biology]. All rights reserved.
- Published
- 2005
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