1. Detecting Zn(II) Ions in Live Cells with Near-Infrared Fluorescent Probes.
- Author
-
Fang M, Xia S, Bi J, Wigstrom TP, Valenzano L, Wang J, Tanasova M, Luck RL, and Liu H
- Subjects
- HeLa Cells, Humans, Microscopy, Fluorescence, Amines chemistry, Amines pharmacology, Carbocyanines chemistry, Carbocyanines pharmacology, Fluorescent Dyes chemistry, Fluorescent Dyes pharmacology, Picolinic Acids chemistry, Picolinic Acids pharmacology, Zinc metabolism
- Abstract
Two near-infrared fluorescent probes ( A and B ) containing hemicyanine structures appended to dipicolylamine (DPA), and a dipicolylamine derivative where one pyridine was substituted with pyrazine, respectively, were synthesized and tested for the identification of Zn(II) ions in live cells. In both probes, an acetyl group is attached to the phenolic oxygen atom of the hemicyanine platform to decrease the probe fluorescence background. Probe A displays sensitive fluorescence responses and binds preferentially to Zn(II) ions over other metal ions such as Cd
2+ ions with a low detection limit of 0.45 nM. In contrast, the emission spectra of probe B is not significantly affected if Zn(II) ions are added. Probe A possesses excellent membrane permeability and low cytotoxicity, allowing for sensitive imaging of both exogenously supplemented Zn(II) ions in live cells, and endogenously releases Zn(II) ions in cells after treatment of 2,2-dithiodipyridine., Competing Interests: The authors declare no conflict of interest.- Published
- 2019
- Full Text
- View/download PDF