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1. [Introduction of aminoalkyl groups into prepared synthetic oligodeoxyribonucleotides by transamination of cytosine residues].

Author

Karpyshev NN, Bondarenko TIu, Vtorushina IA, and Kipriianov SM

Subjects
Alkylation, Base Sequence, Biotin metabolism, Electrophoresis, Polyacrylamide Gel, Molecular Sequence Data, Amines chemistry, Cytosine chemistry, Oligodeoxyribonucleotides chemistry
Abstract

The bisulphite-catalysed transamination of cytosine residues by means of ethylenediamine generally used for the natural nucleic acids modification has been extended on relatively short synthetic oligonucleotides. One of the aminoalkyloligonucleotides thus obtained has been used for preparing a biotinylated hybridisation probe.

Published
1991

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