1. Efficient Biocatalytic Reductive Aminations by Extending the Imine Reductase Toolbox.
- Author
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Roiban, Gheorghe‐Doru, Kern, Marcelo, Liu, Zhi, Hyslop, Julia, Tey, Pei Lyn, Levine, Matthew S., Jordan, Lydia S., Brown, Kristin K., Hadi, Timin, Ihnken, Leigh Anne F., and Brown, Murray J. B.
- Subjects
TERTIARY amines ,CHEMICAL reduction ,BIOCATALYSIS ,CHIRALITY ,NUCLEOPHILIC reactions - Abstract
Chiral secondary and tertiary amines are ubiquitous in pharmaceutical, fine, and specialty chemicals, but their synthesis typically suffers from significant sustainability and selectivity challenges. Biocatalytic alternatives, such as enzyme-catalyzed reductive amination, offer several advantages over traditional chemistry, but industrial applicability has not yet been demonstrated. Herein, we report the use of cell lysates expressing imine reductases operating at 1:1 stoichiometry for a variety of amines and carbonyls. A collection of biocatalysts with diversity in coverage of small molecules and direct industrial applicability is presented. [ABSTRACT FROM AUTHOR]
- Published
- 2017
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