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Different methoxy indolalkylamines based on a common structure, and differing in the side chain attached at the 2 position of the indole ring were studied as MAO A inhibitors. Some are acetylenic derivatives and consequently might behave as "suicide" MAO inhibitors (FA 42, FA 43, FA 45). The rest of compounds are the corresponding parent amines and they might behave as MAO substrates (FA 51, FA 52, FA 53, FA 54). The kinetic behaviour of the parent amines as MAO A and MAO B inhibitors and substrates was determined. In case of acetylenic derivatives, kinetic constants defining the non-covalent adduct formation and the covalent adduct formation were also calculated for the mechanism-based inhibition. These parameters will allow us to establish the correlation with structural features that predetermine one compound to be a good MAO substrate or a good MAO A and MAO B inhibitor.

It has been accepted that, as required mechanistically, the neutral form of the amine is the substrate for monoamine oxidase, despite the amine p K a of above 9.5. The pH dependence of the kinetic parameters for kynuramine oxidation by purified human MAO-A and for phenylethylamine oxidation by MAO-B in granulocytes at pH values from 5 to 10 was consistent with the protonated amine being used. Deprotonation of a group of p K a = 7.1 in MAO-B and p K a = 7.5 ± 0.1 ( n = 4) in MAO-A was important for efficient catalysis. The Ki values for two oxazolidinone inhibitors of MAO-A gave opposite pH-dependence indicating that the uncharged form of each inhibitor bound better than the charged form. Decreased pH induced a blue shift in the spectral maximum of MAO-A indicative of a more hydrophobic environment around the flavin, and also influenced the redox properties of the flavin. [ABSTRACT FROM AUTHOR]