1. Attempting to synthesize lasso peptides using high pressure
- Author
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Łukasz Jaremko, Priyadharshni Rajagopal, Mateusz Waliczek, Amanda Sroczyńska, Monika Kijewska, Magdalena Wierzbicka, Maciej Arkuszewski, Kacper Szczepski, Piotr Stefanowicz, and Mariusz Jaremko
- Subjects
Protein Conformation ,Peptide ,01 natural sciences ,Biochemistry ,Physical Chemistry ,Mass Spectrometry ,Analytical Chemistry ,chemistry.chemical_compound ,Protein structure ,Spectrum Analysis Techniques ,Chemical Equilibrium ,Lasso (statistics) ,Peptide synthesis ,Disulfides ,Post-Translational Modification ,Peptide sequence ,chemistry.chemical_classification ,Liquid Chromatography ,Multidisciplinary ,Chemistry ,Organic Compounds ,Physics ,Chromatographic Techniques ,Classical Mechanics ,Chemical Synthesis ,Solutions ,High pressure ,Physical Sciences ,Medicine ,Engineering and Technology ,Oxidation-Reduction ,Signal Peptides ,Research Article ,Signal peptide ,Biosynthetic Techniques ,Science ,Liquid Chromatography-Mass Spectrometry ,010402 general chemistry ,Research and Analysis Methods ,Pressure ,Amino Acid Sequence ,Peptide Synthesis ,010405 organic chemistry ,Mechanical Engineering ,Organic Chemistry ,Chemical Compounds ,Biology and Life Sciences ,Proteins ,Oxidation reduction ,Combinatorial chemistry ,Amides ,Pistons ,0104 chemical sciences ,Cyclization ,Peptides ,High Pressure - Abstract
Lasso peptides are unique in that the tail of the lasso peptide threads through its macrolactam ring. The unusual structure and biological activity of lasso peptides have generated increased interest from the scientific community in recent years. Because of this, many new types of lasso peptides have been discovered. These peptides can be synthesized by microorganisms efficiently, and yet, their chemical assembly is challenging. Herein, we investigated the possibility of high pressure inducing the cyclization of linear precursors of lasso peptides. Unlike other molecules like rotaxanes which mechanically interlock at high pressure, the threaded lasso peptides did not form, even at pressures the high pressure up to 14 000 kbar.
- Published
- 2020