1. Lipid accumulation by Rhodococcus rhodochrous grown on glucose.
- Author
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Shields-Menard, Sara, Amirsadeghi, Marta, Sukhbaatar, Badamkhand, Revellame, Emmanuel, Hernandez, Rafael, Donaldson, Janet, and French, W.
- Subjects
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RHODOCOCCUS rhodochrous , *BACTERIAL growth , *PHYSIOLOGICAL effects of lipids , *BIOACCUMULATION , *BIODIESEL fuels , *ALTERNATIVE fuels , *FEEDSTOCK , *BIOREMEDIATION - Abstract
Biodiesel is an alternative fuel made from costly vegetable oil feedstocks. Some microorganisms can accumulate lipids when nutrients are limited and carbon is in excess. Rhodococcus rhodochrous is a gram-positive bacterium most often used in bioremediation or acrylamide production. The purpose of this study was to investigate and characterize the lipid accumulation capabilities of R. rhodochrous. Shake flasks and a large-scale fermentation were used to cultivate R. rhodochrous in varying concentrations of glucose. R. rhodochrous achieved almost 50 % of dry cell mass as lipid when grown in 20 g/L of glucose. Wax esters and triglycerides were identified in R. rhodochrous lipid extract. The transesterified extractables of R. rhodochrous consisted of mostly palmitic (35 %) and oleic (42 %) acid methyl esters. This study shows R. rhodochrous to be an oleaginous bacterium with potential for application in alternative fuels. [ABSTRACT FROM AUTHOR]
- Published
- 2015
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