1. Establishment of adult rat Schwann cell cultures: effect of b-FGF, alpha-MSH, NGF, PDGF, and TGF-beta on cell cycle.
- Author
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Peulve P, Laquerriere A, Paresy M, Hemet J, and Tadie M
- Subjects
- Age Factors, Animals, Cell Cycle drug effects, Cells, Cultured, Cholera Toxin pharmacology, Culture Techniques methods, Fibroblast Growth Factor 2 pharmacology, Flow Cytometry, Immunohistochemistry, Microscopy, Phase-Contrast, Nerve Growth Factors pharmacology, Platelet-Derived Growth Factor pharmacology, Rats, Rats, Wistar, Schwann Cells cytology, Schwann Cells drug effects, Sciatic Nerve cytology, Transforming Growth Factor beta pharmacology, Cell Cycle physiology, Growth Substances pharmacology, Schwann Cells physiology, alpha-MSH pharmacology
- Abstract
Mitotically active Schwann cells isolated from adult rat sciatic nerve segments were cultivated, using a bivariate BrdU/DNA flow cytometry analysis, to test the effect of b-FGF (10 ng/ml), alpha-MSH (10 ng/ml), NGF (10 ng/ml), PDGF-AB (10 ng/ml), and TGF-beta 1 (10 ng/ml) on the cell cycle. Compared to control or cholera toxin-treated cultures, no significant differences (P < 0.05; Newmann-Keuls test) were observed in the proportion of G0G1 cells, S cells, G2M cells, and in the LI when alpha-MSH, NGF, PDGF-AB, or TGF-beta 1 were present. The S phase duration varied from 6.16 +/- 0.24 to 7.86 +/- 2.6 h, and the deduced potential doubling time was estimated at between 46.70 +/- 7.09 and 56.05 +/- 7.55 h. In contrast, when b-FGF was added to the culture, the cell cycle was significantly modified, and the proportion of G0G1 cells decreased from 68-77% to 59-64%, while the proportion of S cells increased from 14-16% to 24.0-26.4%. Although S phase duration was not significantly changed (6.02 +/- 0.36 h), the 1.7- to 2.8-fold LI increase reduced the potential doubling time to 25.99 +/- 6.13 h. We conclude from these results that only b-FGF-induced adult rat Schwann cells dramatically reenter in cell cycle and that this growth factor may be an axonally derived signal-promoting mitogenesis after nerve injury.
- Published
- 1994
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