1. Rapid microsatellite development in Gekko japonicus using sequenced restriction-site associated DNA markers
- Author
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Zhou Hb, Ping J, Zhang Yp, Li Lm, L. Wei, and Shao Ww
- Subjects
Genetic Markers ,China ,Linkage disequilibrium ,Restriction Mapping ,Population ,Locus (genetics) ,Linkage Disequilibrium ,Gekko ,Genetics ,Animals ,education ,Molecular Biology ,Alleles ,Genetic diversity ,education.field_of_study ,Polymorphism, Genetic ,biology ,Restriction site associated DNA markers ,Genetic Variation ,Lizards ,General Medicine ,biology.organism_classification ,Genetics, Population ,Genetic marker ,Microsatellite ,Microsatellite Repeats - Abstract
Twelve polymorphic microsatellite loci were isolated in the Japanese gecko, Gekko japonicus. We genotyped one population from Wenzhou, Zhejiang Province, China (N = 36). The mean number of observed alleles per locus was 7.3 (range 4 to 13). Observed and expected heterozygosity values ranged from 0.200 to 0.944 and from 0.395 to 0.797, respectively. One locus (GJ20) showed significant departure from Hardy-Weinberg equilibrium; no linkage disequilibrium was found between any two loci. These informative microsatellite markers will be useful for population genetic analyses of G. japonicus and other species in the genus Gekko.
- Published
- 2015
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