1. Novel Polymorphic Microsatellite Loci for the Korean Black Scraper (Thamnaconus modestus), and Their Application to the Genetic Characterization of Wild and Farmed Populations
- Author
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Eun Mi Kim, Bai Ik Lee, Chun Mae Dong, Jang Wook Lee, Yi Cheong Kim, and Hye Suck An
- Subjects
Genotype ,ved/biology.organism_classification_rank.species ,Population ,Zoology ,Aquaculture ,Korean black scraper ,Catalysis ,Inorganic Chemistry ,lcsh:Chemistry ,Gene Frequency ,Republic of Korea ,Genetic variation ,genetic variability ,Technical Note ,Animals ,Polymorphic Microsatellite Marker ,Genetic variability ,Physical and Theoretical Chemistry ,Black scraper ,education ,Molecular Biology ,lcsh:QH301-705.5 ,Alleles ,Spectroscopy ,Gene Library ,Genetics ,education.field_of_study ,Polymorphism, Genetic ,Stephanolepis cirrhifer ,biology ,Tetraodontiformes ,ved/biology ,Organic Chemistry ,Genetic Variation ,General Medicine ,microsatellite loci ,biology.organism_classification ,Computer Science Applications ,Thamnaconus modestus ,Genetics, Population ,lcsh:Biology (General) ,lcsh:QD1-999 ,Genetic Loci ,Genetic marker ,Microsatellite ,genetic marker ,Microsatellite Repeats - Abstract
In this study, we developed 20 polymorphic microsatellite markers for the Korean black scraper, Thamnaconus modestus (Günther, 1877), Monacanthidae, and used them to compare allelic variation between wild and hatchery populations in Korea. All loci were readily amplified and demonstrated allelic variability, with the number of alleles ranging from 5–35 in the wild population and 5–22 in the farmed population. The average observed and expected heterozygosities were estimated, respectively, as 0.74 and 0.80 in the hatchery samples and 0.78 and 0.81 in the wild ones. These results indicate lower genetic variability in the hatchery population than in the wild population and minor, but significant, genetic differentiation between the two populations (FST = 0.005, P < 0.01). Additionally, cross-amplification was tested in another monacanthid species, Stephanolepis cirrhifer; many loci were found that yielded useful information. The high degree of polymorphism exhibited by the 20 microsatellites will be useful in future aquaculture and population genetic studies for developing conservation and management plans.
- Published
- 2011