1. A serine-substituted P450 catalyzes highly efficient carbene transfer to olefins in vivo.
- Author
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Coelho PS, Wang ZJ, Ener ME, Baril SA, Kannan A, Arnold FH, and Brustad EM
- Subjects
- Alkenes chemistry, Biocatalysis, Cytochrome P-450 Enzyme System chemistry, Cytochrome P-450 Enzyme System genetics, Escherichia coli chemistry, Escherichia coli cytology, Escherichia coli metabolism, Methane chemistry, Methane metabolism, Mutation, Serine chemistry, Alkenes metabolism, Cytochrome P-450 Enzyme System metabolism, Methane analogs & derivatives, Serine metabolism
- Abstract
Whole-cell catalysts for non-natural chemical reactions will open new routes to sustainable production of chemicals. We designed a cytochrome 'P411' with unique serine-heme ligation that catalyzes efficient and selective olefin cyclopropanation in intact Escherichia coli cells. The mutation C400S in cytochrome P450(BM3) gives a signature ferrous CO Soret peak at 411 nm, abolishes monooxygenation activity, raises the resting-state Fe(III)-to-Fe(II) reduction potential and substantially improves NAD(P)H-driven activity.
- Published
- 2013
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