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Your search keyword '"Polyakova TY"' showing total 5 results
Start Over Author "Polyakova TY" Topic alkaloids
5 results on '"Polyakova TY"'

1. Role of JAK/STAT3 Signaling in Functional Stimulation of Mesenchymal Progenitor Cells with Alkaloid Songorine.

Author

Zyuz'kov GN, Udut EV, Miroshnichenko LA, Polyakova TY, Simanina EV, Stavrova LA, Prosekin GA, Zhdanov VV, and Udut VV

Subjects
Alkaloids isolation & purification, Animals, Anthraquinones pharmacology, Cell Differentiation drug effects, Cell Proliferation drug effects, Fibroblast Growth Factors pharmacology, Gene Expression Regulation, Janus Kinases antagonists & inhibitors, Janus Kinases metabolism, Mesenchymal Stem Cells cytology, Mesenchymal Stem Cells metabolism, Mice, Mice, Inbred C57BL, Nitriles, Phosphorylation drug effects, Plant Extracts chemistry, Primary Cell Culture, Pyrazoles pharmacology, Pyrimidines, STAT3 Transcription Factor antagonists & inhibitors, STAT3 Transcription Factor metabolism, Signal Transduction, Sulfonamides pharmacology, Aconitum chemistry, Alkaloids pharmacology, Janus Kinases genetics, Mesenchymal Stem Cells drug effects, STAT3 Transcription Factor genetics
Abstract

JAK/STAT signaling pathway was examined comparatively during realization of growth potential of mesenchymal progenitor cells stimulated with diterpene alkaloid songorine or fibroblast growth factor. The stimulating role of JAKs and STAT3 on the mitotic activity and differentiation of progenitor cells cultured with songorine was revealed. Under these conditions, the study demonstrated suppression of fibroblast colony formation against the background of reduced number of actively proliferating CFU-fibroblasts and a drop of differentiation index of progenitor cells induced by pan-JAKs and STAT3 inhibitors. The observed changes were in almost complete agreement with the character of functional reactions of the progenitor elements in response to blockade of JAKs and STAT3 with fibroblast growth factor. In addition, blockade of JAKs with this factor enhanced the differentiation rate of the progenitor cells.

Published
2018
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2. Role of JAK1, JAK2, and JAK3 in Functional Stimulation of Mesenchymal Precursor Cells by Alkaloid Songorine.

Author

Zyuz'kov GN, Udut EV, Miroshnichenko LA, Simanina EV, Polyakova TY, Stavrova LA, Udut VV, Minakova MY, Dygai AM, Chaikovskii AV, Agafonov VI, and Zhdanov VV

Subjects
Animals, Cell Differentiation drug effects, Humans, Janus Kinase 1 genetics, Janus Kinase 2 genetics, Janus Kinase 3 genetics, Mesenchymal Stem Cells drug effects, Mesenchymal Stem Cells metabolism, Protein Kinase Inhibitors pharmacology, Regenerative Medicine methods, Signal Transduction physiology, Stem Cells cytology, Stem Cells metabolism, Alkaloids pharmacology, Janus Kinase 1 metabolism, Janus Kinase 2 metabolism, Janus Kinase 3 metabolism
Abstract

We studied the role of some JAK in the effect of diterpene alkaloid songorine on realization of the growth potential of mesenchymal precursor cells. The participation of JAK1, JAK2, and JAK3 in stimulation of proliferation of the precursor cells was demonstrated. Specific inhibitors of these JAK reduced the yield of fibroblast CFU and the rate of their division. Inhibition of JAK2 against the background of songorine treatment increased the rate of precursor differentiation.

Published
2017
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3. Psychopharmacological Effects of Alkaloid Z77 under Conditions of Posthypoxic Encephalopathy and Mechanisms of Their Development.

Author

Zyuz'kov GN, Losev EA, Chaikovskii AV, Suslov NI, Zhdanov VV, Udut EV, Miroshnichenko LA, Simanina EV, Polyakova TY, Povet'eva TN, Nesterova YV, Stavrova LA, Udut VV, Minakova MY, and Dygai AM

Subjects
Animals, Brain drug effects, Exploratory Behavior drug effects, Male, Mice, Neural Stem Cells drug effects, Regenerative Medicine, Alkaloids pharmacology, Alkaloids therapeutic use, Brain Diseases drug therapy
Abstract

Psychopharmacological effects of atisine-type diterpene alkaloid Z77 were studied under conditions of experimental posthypoxic encephalopathy. The preparation had a pronounced cerebroprotective effect consisting in normalization of orientation and exploratory behavior and conditioned activity in experimental animals. These changes were accompanied by significant increase in the number of neural stem cells in the paraventricular region of the brain and markedly enhanced production of neurotrophic growth factors by neural tissue microenvironment cells.

Published
2016
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4. Role of JNK and Involvement of p53 in Stimulation of Growth Potential Realization of Mesenchymal Precursor Cells by Alkaloid Songorine.

Author

Zyuz'kov GN, Zhdanov VV, Udut EV, Miroshnichenko LA, Chaikovskii AV, Simanina EV, Udut VV, Stavrova LA, Polyakova TY, Minakova MY, Trifonova OY, Gridneva TD, and Dygai AM

Subjects
Animals, Apoptosis drug effects, Benzothiazoles pharmacology, Cells, Cultured, Colony-Forming Units Assay, JNK Mitogen-Activated Protein Kinases antagonists & inhibitors, MAP Kinase Signaling System physiology, Male, Mesenchymal Stem Cells metabolism, Mice, Mice, Inbred CBA, Protein Kinase Inhibitors pharmacology, Toluene analogs & derivatives, Toluene pharmacology, Tumor Suppressor Protein p53 antagonists & inhibitors, Alkaloids pharmacology, MAP Kinase Signaling System drug effects, Mesenchymal Stem Cells drug effects, Tumor Suppressor Protein p53 physiology
Abstract

The role of JNK-mediated signal pathway and participation of p53 transcription factor in stimulation of realization of the growth potential of the mesenchymal precursor cells by alkaloid songorine were examined in vitro. Specific inhibitors of JNK and p53 enhanced stimulation of fibroblast colony/cluster formation and proliferative activity of mesenchymal precursor cells. Under these conditions, more pronounced effects were observed with early precursors of fibroblast CFU and in both cases were accompanied by a decrease in differentiation index of progenitor elements.

Published
2015
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5. Role of cAMP- and IKK-2-Dependent Signaling Pathways in Functional Stimulation of Mesenchymal Progenitor Cells with Alkaloid Songorine.

Author

Zyuz'kov GN, Zhdanov VV, Udut EV, Miroshnichenko LA, Chaikovskii AV, Simanina EV, Polyakova TY, Minakova MY, Udut VV, Tolstikova TG, Shul'ts EE, Stavrova LA, Burmina YV, Suslov NI, and Dygai AM

Subjects
Adenylyl Cyclases metabolism, Animals, Cell Differentiation drug effects, Cell Proliferation drug effects, Cyclic AMP antagonists & inhibitors, Dideoxyadenosine analogs & derivatives, Dideoxyadenosine pharmacology, Enzyme Inhibitors pharmacology, Gene Expression Regulation, I-kappa B Kinase antagonists & inhibitors, I-kappa B Kinase metabolism, Male, Mesenchymal Stem Cells cytology, Mesenchymal Stem Cells metabolism, Mice, Mice, Inbred CBA, Primary Cell Culture, Signal Transduction, Stem Cells, Thiophenes pharmacology, Adenylyl Cyclases genetics, Alkaloids pharmacology, Cyclic AMP metabolism, I-kappa B Kinase genetics, Mesenchymal Stem Cells drug effects
Abstract

The role of cAMP- and IKK-2-dependent pathways in stimulation of the growth capacity of mesenchymal progenitor cells with alkaloid songorine was studied in vitro. Inhibitors of adenylate cyclase and IKK-2 were shown to abolish the increase in proliferative activity of progenitor cells. Moreover, blockade of the inhibitory kinase complex was accompanied by a decrease in the intensity of progenitor cell differentiation.

Published
2015
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