1. Transcriptomics comparison reveals the diversity of ethylene and methyl-jasmonate in roles of TIA metabolism in <italic>Catharanthus roseus</italic>.
- Author
-
Pan, Ya-jie, Lin, Ying-chao, Yu, Bo-fan, Zu, Yuan-gang, Yu, Fang, and Tang, Zhong-Hua
- Subjects
- *
CATHARANTHUS roseus , *ALKALOIDS , *ETHYLENE , *ATP-binding cassette transporters , *TRANSCRIPTOMES - Abstract
Background: The medicinal plant,
Catharanthus roseus (C. roseus ), accumulates a wide range of terpenoid indole alkaloids (TIAs). Ethylene (ET) and methyl-jasmonate (MeJA) were previously reported as effective elicitors for the production of various valuable secondary metabolites ofC. roseus , while a few ET or MeJA induced transcriptomic research is yet reported on this species. In this study, the de-novo transcriptome assembly ofC. roseus is performed by using the next-generation sequencing technology. Results: The result shows that phenolic biosynthesis genes respond specifically to ET in leaves, monoterpenoid biosynthesis genes respond specifically to MeJA in roots. By screening the database, 23 ATP-binding cassette (ABC) transporter partial sequences are identified inC. roseus . On this basis, more than 80 key genes that encode key enzymes (namely TIA pathway, transcriptional factor (TF) and candidate ABC transporter) of alkaloid synthesis in TIA biosynthetic pathways are chosen to explore the integrative responses to ET and MeJA at the transcriptional level. Our data indicated that TIA accumulation is strictly regulated by the TF ethylene responsive factor (ERF) and bHLH iridoid synthesis 1 (BIS1). The heatmap, combined with principal component analysis (PCA) ofC. roseus, shows thatERF co-expression withABC2 andABC8 specific expression in roots affect the root-specific accumulation of vinblastine inC. roseus . On the contrast,BIS1 activities follow a similar pattern ofABC3 andCrTPT2 specific expression in leaves, which affects the leaf-specific accumulation of vindoline inC. roseus . Conclusions: Results presented above illustrate that ethylene has a stronger effect than MeJA on TIA induction at both transcriptional and metabolite level. Furthermore, meta-analysis reveals that ERF and BIS1 form a positive feedback loop connecting two ABC transporters respectively and are actively involved in TIAs responding to ET and MeJA inC. roseus . [ABSTRACT FROM AUTHOR]- Published
- 2018
- Full Text
- View/download PDF