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Start Over Descriptor "Transposons -- Chemical properties" Topic alkaline phosphatase -- chemical properties
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1. In vivo insertional mutagenesis in Corynebacterium pseudotuberculosis: An efficient means to identity DNA sequences encoding exported proteins

Author

Dorrela, Fernanda A., Miyoshi, Anderson, Azevedo, Vasco, Estevam, Estela M., Meyer, Roberto, Pacheco, Luis G.C., Oliveira, Sergio C., Guimaraes, Claudia T., Barsante, Michele M., Lana, Ubiraci G.P., and Gomes, Eliane A.

Subjects
Corynebacteria -- Genetic aspects, Mutagenesis -- Research, Transposons -- Chemical properties, Transposons -- Structure, Alkaline phosphatase -- Chemical properties, Biological sciences
Abstract

Exported proteins of the animal pathogen Corynebacterium pseudotuberculosis is identified using the reporter transposon-based system TnFuZ. Results revealed that thirty-four out of 1,500 mutants had detectable alkaline phosphatase (PhoZ) activity.

Published
2006

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