1. Genome-scale deconvolution of RNA structure ensembles.
- Author
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Morandi E, Manfredonia I, Simon LM, Anselmi F, van Hemert MJ, Oliviero S, and Incarnato D
- Subjects
- 3' Untranslated Regions genetics, COVID-19, Humans, Mutation drug effects, Mutation genetics, RNA, Viral genetics, Sulfuric Acid Esters pharmacology, Algorithms, Genome, Viral genetics, Nucleic Acid Conformation, RNA, Viral chemistry, SARS-CoV-2 genetics
- Abstract
RNA structure heterogeneity is a major challenge when querying RNA structures with chemical probing. We introduce DRACO, an algorithm for the deconvolution of coexisting RNA conformations from mutational profiling experiments. Analysis of the SARS-CoV-2 genome using dimethyl sulfate mutational profiling with sequencing (DMS-MaPseq) and DRACO, identifies multiple regions that fold into two mutually exclusive conformations, including a conserved structural switch in the 3' untranslated region. This work may open the way to dissecting the heterogeneity of the RNA structurome.
- Published
- 2021
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