1. Alginate/chitosan microcapsules for in-situ delivery of the protein, interleukin-1 receptor antagonist (IL-1Ra), for the treatment of dextran sulfate sodium (DSS)-induced colitis in a mouse model.
- Author
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Cao J, Cheng J, Xi S, Qi X, Shen S, and Ge Y
- Subjects
- Animals, Capsules, Colitis pathology, Dextran Sulfate, Disease Models, Animal, Drug Carriers chemistry, Drug Delivery Systems, Drug Liberation, Female, Hydrogen-Ion Concentration, Interleukin 1 Receptor Antagonist Protein pharmacology, Interleukin-1beta metabolism, Mice, Mice, Inbred BALB C, Treatment Outcome, Tumor Necrosis Factor-alpha metabolism, Alginates chemistry, Chitosan chemistry, Colitis drug therapy, Interleukin 1 Receptor Antagonist Protein administration & dosage
- Abstract
Targeted delivery of bioactive compounds such as proteins to the colon has numerous advantages for the therapeutic treatment of inflammatory bowel disease. The present study sought to fabricate alginate/chitosan microcapsules containing IL-1Ra (Alg/Chi/IL-1Ra MC) via a single-step electrospraying method. Two important factors of efficacy were measured-the pH-responsiveness of the microcapsule and the in-vitro drug release profile. The DSS-induced colitis mouse model was used to evaluate the therapeutic effect of the Alg/Chi/IL-1Ra microcapsules, with results showing the protective effect of the Alg/Chi microcapsules for the passage of IL-1Ra through the harsh environment of the upper gastrointestinal tract. This effect was owing to the pH-sensitive response of the microcapsule, which allowed the targeted release of IL-1Ra in the colon. DAI evaluation, colon length, colon tissue morphology, histologic damage scores and relative protein concentrations (MPO, TNF-α and IL-1β) demonstrated that the Alg/Chi/IL-1Ra microcapsules alleviated DSS-induced colitis in mice. The present study thus demonstrates a practical means of oral delivery of proteins, in-situ colon release, and a promising application of IL-1Ra in the treatment of autoimmune and inflammatory diseases., (Copyright © 2019 Elsevier B.V. All rights reserved.)
- Published
- 2019
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