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‘Algae Service for LIFE’, the project supported by the European Union, seeks to promote best practices in ecological service and the circular economy by implementing innovative complex system of three interlinked elements: i) prototypes for harvesting of cyanobacteria and macroalgae biomass; ii) distant methods for surveying of the blooms and defining hot-spots of algal agglomerations; and iii) restitution of harvesting costs by redesigning of waste algal biomass into valuable products. The current paper describes application of algal biomass part of the project by providing actions in redesigning of harvested waste biomass of cyanobacteria and macroalgae into potential valuable products for sustainable management and recycling of environmental resources. It also highlights the socio-economic aspects of the project and added value of the project for the European Union.

Polyphenols are chemicals that primarily inhibit the growth of various autotrophic organisms. The presence of these metabolites greatly boosts the ecological dominance of eg. Cladophora, which creates large surface mats. The main goal of our work was to quantify the phenol and polyphenols (allelopathic substances) secreted by the macroalgae as a result of exposure to biotic stress caused by competition. The research was carried out on the Cladophora glomerata biomass collected from two freshwater ecosystems located in Wielkopolska Region (Poland, Fig. 1): Oporzynskie Lake (N52° 55′, E 17° 9′) and Nielba River (N52˚48′, E 17° 12′). Seasonal variability (May-October 2015) in the properties of C. glomerata mats in the river and lake ecosystem was also analyzed in relation to the physicochemical parameters of water. In addition, the content of pigments in the analyzed biomass was determined during the appearance of algae mats in water reservoirs. Biomass extraction was performed to determine the phenolic and carotenoid contents (chlorophyll and carotenoids) by using two extraction methods: microwave-assisted extraction (MAE) and supercritical fluid extraction (SFE). After isolation of the phenols from the thalli (mats) and the habitat, they were analyzed using the Folin-Ciocalteu method with some modifications, while the pigment content (chlorophyll and carotenoids) was evaluated by the spectrophotometric method Liechtenthaler (1987) with some variations. Analysis of the content of these components in algae extracts indicates that the tendency of changes in their contents was similar or the same. Growth and decrease of phenolic content (Cladophora T MAE and Cladophora T SFE) and pigments (MAE chlorophyll, SFE and MAE carotenoids) at the same time were independent of the insulation method used. The mats formed by C. glomerata on the surface of Lake Oporzynskie were more stable and larger surface area than those on the Nielba River, which could explain differences in polyphenol concentrations in these two aquatic ecosystems. The results suggest a reduction in the secretion of phenolic compounds with an aging population of algae.

The biologically active compounds (fatty acids, pigments, phenolics, and flavonoid content) were studied in supercritical fluid extracts from the biomass of marine (Ulva clathrata, Cladophora glomerata, Polysiphonia fucoides, and their multi-species mixture) and freshwater (C. glomerata) macroalgae. Different extraction techniques were used in order to compare differences in the biologically active compound composition of the macroalgal extracts. The results indicated that the saturated and unsaturated fatty acids ranged from C9:0 to C22:0. The analysis of differences in the composition of unsaturated to saturated fatty acids in extracts showed that palmitic acid (C16:0) and oleic acid (C18:1, n-9) reached the highest value not only in marine monospecies and multi-species biomass but also in the freshwater macroalga C. glomerata. When comparing the similarity between the concentration of fatty acids and the ratio of the concentration of unsaturated fatty acids to saturated in macroalgal extracts, we found small but not statistically significant variations in values between years (up to 10%). This is acceptable for applications as a stable raw material for industrial purposes. Significantly higher values of fatty acids, carotenoids, and chlorophylls were obtained in the case of SC-CO2 extraction. The active ingredients of polyphenols, possessing antioxidant activity ranged from approximately 2–4%. Moreover, flavonoids represented less than 10% of the total content of polyphenolic compounds. The extraction efficiency of polyphenols was higher from a mixture of marine algae for the ultrasound-assisted extraction compared to freshwater. All these findings show that marine and freshwater macroalgae, as a raw material, have the optimal biologically active compounds composition for cosmetics.

For the first time polysaccharidic ulvans have been isolated from freshwater green macroalgae Ulva flexuosa and Cladophora glomerata. One of the main components of ulvan is 3-sulfated rhamnoglucuronan showing a wide range of interesting properties and being a source of rare sugars. The production of mono- and oligo-saccharides from this polysaccharide can potentially be applied in cosmetic, pharmaceutical and food industry. In this paper a new and effective method for the isolation of ulvans from freshwater algae is described. The substance has been characterized after enzymatic hydrolysis by Fourier Transform Infrared (FT-IR) spectroscopy and elemental analysis. FT-IR spectroscopy permits identification of the band characteristic of the sulfonic acid groups, specific for this kind of polysaccharides. With increasing size of the thalli, the amount of ulvans in the algae cells increases. Hence, freshwater green macroalgae U. flexuosa and C. glomerata were found as a new source of polysaccharidic ulvans that can be considered to be potentially used in medicine, pharmacy, food and cosmetic industry.

Water samples from the different basins of the Baltic Sea and North Sea were collected during four cruises taken place in 2016–2017. Altogether 60 water samples for 3H activity concentration and 120 water samples for stable isotopes ratio (δ18O and δ2H) were analysed. Exceptionally strong inflow activity from North Sea to the Baltic Sea occurred prior our surveys. The data obtained enabled comprehensive evaluation of tritium and stable isotopes distribution in waters of Baltic Sea and contributing inputs, including riverine and atmospheric freshwater flux and saline water inflow through the Danish Straits. By comparing with the previous studies, we noticed a tendency toward an increase in the δ18O and δ2H values of zero-salinity component over time, which may be due to climate change. In the case of tritium, the influence of artificial sources has also been noticed., Europos Sąjungos LIFE programos projektas „Algae Service for LIFE“ skatina gerąją ekologinių paslaugų praktiką ir žiedinės ekonomikos kūrimą. Projekto tikslas yra demonstruoti integruotą efektyvų maistinių medžiagų ir dumblių „žydėjimų“ valdymą upių baseinų lygyje, surenkant perteklinę melsvabakterių ir makrodumblių biomasę iš skirtingų vandens telkinių (upių, ežerų, tvenkinių, Kuršių marių). Taip pat projektu siekiama skleisti informaciją nacionalinėms ir vietos valdžios institucijoms, verslo bendruomenei bei didinti visuomenėssusidomėjimą vandens telkinių ekologine būkle. Straipsnyje aptariamos ekologinės vandens „žydėjimų“ kontrolės priemonės ir paslaugos, akcentuojant gėlavandenių ekosistemų eutrofikacijos ir „žydėjimų“ priežastis ir pasekmes. Siūlomos projekte priemonės prisideda prie Europos Sąjungos strateginių direktyvų įgyvendinimo.

Potential factors affecting the occurrence of filamentous algae include the morphometry of water bodies, the type of substrate and physicochemical conditions of the habitat. This study attempts to describe the individual filamentous algae species recorded in Poland, taking into account the trophic and ecological characteristics based on our own research and available literature data. Filamentous algae attached to the substrate as well as those forming free-floating patches (loose or dense mats) and crusts occur in all types of water bodies. Among the representatives of filamentous algae species, Cladophora have the greatest time-spatial range for they grow abundantly in all types of water bodies. In other parts of Poland, Cladophora species are most common in rivers and shallow lakes. In the typical filamentous algae community, Cladophora, Spirogyra and Oedogonium occur with the highest frequency; Zygnema, Mougeotia, Microspora and Rhizoclonium are also present, but with smaller frequency. According to our studies, distribution of filamentous algae species is correlated with the eutrophic index (PO43 -, N-NO3 -, Chl a), chlorides of anthropogenic origin and TDS.

Biomass of marine algae and their extracts are now one of the most widely used natural ingredients in cosmetics, because of the presence of a wide variety of biologically active compounds in their thalli. Mainly, it comes down to marine species, but the most current interdisciplinary research shows that freshwater macroscopic green algae species (e.g., Chara fragilis, Cladophora glomerata, Ulva flexuosa) may also be a rich source of macro- and micronutrients and other bioactive substances such as fatty acids, polysaccharides, pigments, polyphenols, etc. Freshwater macroalgae are a rare object of study and are practically absent within the cosmetics market. In this chapter, algae harvesting and the processing of algal biomass are discussed. This chapter shows, in particular, that the presence of bioactive substances in their thalli determines the broad biological activity and potential use of freshwater algae in the production of cosmetics.

Biomass derived from algae is a valuable raw product for agriculture and the chemical industry. The chemical composition of the algae biomass obtained from the natural environment, culture under natural conditions, and culture in bioreactors determines its application to the energy, fuel, and cosmetic industries. The use of biomass, as well as the extracts derived from biomass, is discussed in regard to the economic aspect and life-cycle assessment. The economic aspects of obtaining biomass algae in the product life cycle are discussed for the bioproducts industry.

The occurrence of algal-cyanobacterial metaphyton mats in field (Konojad village) and artificial ponds (Poznań city) was investigated in the Wielkopolska region. The surface area of algal mats ranged between ca. 45% (natural pond) and 10% (artificial pond) of the total water surface, and the mats occurred from the water surface to the bottom; the algal diversity was similar in both types of ponds, i.e. about 10 taxa. The most frequently recorded and dominant taxa in small water bodies were: Spirogyra spp., Cladophora fracta in the artificial pond and Oedogonium spp., Cladophora rivularis in the natural pond. The dynamics of macroalgae demonstrated a pattern comprising different phases of taxa dominance. For example, April regarded as the first phase was characterized by a small amount of metaphyton, represented by Tribonema and Ulothrix taxa. In May, these species disappeared and were replaced by: Oedogonium spp. and Cladophora spp. which formed large aggregations in the field pond, and Spirogyra spp. which abundantly proliferated in the artificial pond. From June, however, Oedogonium and/or Cladophora significantly dominated in the mat structure and at the same time covered a large area of the water surface. The algal community was dominated by common algal taxa, among which single filaments of other taxa could also occur.

Getting to know the response of different groups of aquatic organisms tested in altered thermal environments to environmental conditions makes it possible to understand processes of adaptation and limitation factors such as temperature and light. Field sites were located in three thermally abnormal lakes (cooling system of power plants), in eastern part of Wielkopolska region (western Poland): Pątnowskie, Wąsosko-Mikorzyńskie and Licheńskie. Water temperatures of these lakes do not fall below 10°C throughout the year, and the surface water temperature in spring is about 20˚C. In this study, we investigated the species structure of the spring phytoplankton community in a temperature gradient and analyzed diversity of periphyton collected from alien species (Vallisneria spiralis) and stones. 94 taxa belonging to 56 genera of algae (including phytoplankton and periphyton) were determined. The highest number of algae species were observed among Chlorophyta (49), Bacillariophyceae (34) and Cyanobacteria (6). In spite of important differences in temperature in the investigated lakes, taxonomic composition of phytoplankton was comparable. Thermophilic species: Glochidinium penardiforme and Cylindrospermopsis raciborskii were found in the species structure (blooms were not observed). The obtained data also showed that the biotic surface of Vallisneria spiralis was a better substrate for Bacillariophyceae colonization than stones. The examination in the spring season of these thermally altered lakes, indicated the taxonomic composition of phytoplankton typical for eutrophic reservoirs (not heated). There was no replacement of any phytoplankton groups which are characteristic for spring conditions, even if there were changes in the competition dynamics.

This paper presents the results of ultrastructural studies and ecological aspects of some phytoplankton species belonging to the groups of cyanobacteria (Planktothrix rubescens, Synechocystis aquatilis) and green algae (Desmodesmus grahneisii). Specimens were collected during summertime from the mesotrophic and stratified Lake Holzmaar (Western Germany) as planktonic forms from the pelagic zone. The highest cyanobacterium concentration was detected in the metalimnion, where the alkaline pH, low phosphorus and high nitrogen contents were recorded. P. rubescens was characterized by straight filaments up to 1000 μm long and 5.4-8 μm wide and numerous aerotopes in cells. The accompanying algae were identified by ultrastructural analysis and photographic documentation was provided. In the case of D. grahneisii, chloroplast was situated in the parietal part of cell, with one large, oval pyrenoid and, in addition, the granular and spiny cell wall was an important taxonomical criterium for the Desmodesmus genus. This is in contrast with the cyanobacterium S. aquatilis, in which a homogeneous content with visible chromatoplasma was mostly distributed throughout the cell. This algal association was stable in the epilimnion characterized by the presence of high temperature, pH values (>8), nitrate nitrogen and oxygen concentrations

The paper discusses the potential applicability of the process of supercritical fluid extraction (SFE) in the production of algal extracts with the consideration of the process conditions and yields. State of the art in the research on solvent-free isolation of biologically active compounds from the biomass of algae was presented. Various aspects related with the properties of useful compounds found in cells of microalgae and macroalgae were discussed, including their potential applications as the natural components of plant protection products (biostimulants and bioregulators), dietary feed and food supplements, and pharmaceuticals. Analytical methods of determination of the natural compounds derived from algae were discussed. Algal extracts produced by SFE process enable obtaining a solvent-free concentrate of biologically active compounds; however, detailed economic analysis, as well as elaboration of products standardization procedures, is required in order to implement the products in the market.