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The experiment aimed to screen for high-yielding hybrid combinations of male-sterile alfalfa lines. A total of 10 hybrid combinations were prepared with one alfalfa male sterile line (MS-GN) as the female parent and 10 excellent alfalfa plants (Alfalfa 354-05, Cavalier-02, Gannong NO. 8-04, Alfalfa 440-02, Queen-25, Alfalfa 319-01, Longdong Alfalfa-06, Alfalfa 343-02, Algonkin-19, and Suntory-74) as the male parents, and the numbers were M×1, M×2, M×3, M×4, M×5, M×6, M×7, M×8, M×9, and M×10. The high-yield hybrid combinations were screened by measuring the plant height, stem diameter, primary branch number, fresh yields and hay yields of the F1 generation of the hybrid combination for two consecutive years, combined with the analysis of general compounding ability. The results showed that M×4 had the highest two-year average plant height, which was significantly higher than that of M×7, M×3, M×5, M×8 and M×10 (P<0.05). The two-year average stem diameter of M×6 was the highest, and the two-year average primary branch number of M×1 was the highest. M×1 and M×6 had the highest two-year average hay yield of 2.02 kg/plant, while M×4 and M×1 had the highest two-year average hay yield of 0.60 kg/plant. Plant height, stem diameter, and primary branch number were positively correlated with grass yield, and plant height and primary branch number were significantly or extremely correlated with fresh yield and hay yield (P<0.05 or P<0.01). The cluster analysis of yield traits divided the hybrid combinations into four categories, among which the combinations with better yield traits were M×1, M×6, and M×4, which were consistent with the results of the comprehensive analysis of grey correlation degree. In the general compounding ability (GCA) effect value, M×4 had the highest plant height, M×6 had the highest stem diameter, M×1 had the highest number of first-order branches, M×1 and M×6 had the highest yield, and M×1 and M×4 had the highest hay yield. The results showed that M×1, M×4, and M×6 had better comprehensive performance in yield traits among the hybrid combinations, and they were high-yield hybrid combinations. [ABSTRACT FROM AUTHOR]

In the study, related indexes of production performance and nutritional quality of 15 alfalfa varieties were measured, and comprehensive evaluation of traits were evaluated by grey relational degree analysis, and the feeding value of varieties were compared. The results showed that the fresh grass yield of Gannong NO.6, Gannong NO.8 and WL298HQ was higher than Zhongmu NO.1. The hay yield of Gannong NO.6 was higher than Zhongmu NO.1, and the plant height of Gannong NO.6 was also better. The average yield of fresh yield and hay yield of Gannong NO.6 were increased 18.43% and 13.28% compared with Zhongmu NO. 1. There was no significant difference in DM content among different varieties (P>0.05). Longmu 801 had the highest CP content (20.76%), followed by Yong Shi, 4030, WL298HQ, 7G3, WL-SQT, 42IQ, Gannong NO.8 and Yan Bao, which were significantly higher than that of Zhongmu NO.1 (P<0.05). The results showed that Gannong NO.6, Longmu 801, 7G3, WL298HQ, Gannong NO.8, 42IQ, Yan Bao and Yong Shi were better by grey relational degree analysis. Therefore, the above eight varieties have high feeding value and were suitable for promotion and utilization in mountainous area of Southern Ningxia. [ABSTRACT FROM AUTHOR]