1. Nanogel receptors for high isoelectric point protein detection: influence of electrostatic and covalent polymer–protein interactions
- Author
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Susana P. Simmonds, Eric V. Anslyn, Samuel D. Dahlhauser, James F. Reuther, Jordyn M. Wyse, H. K. H. Jocelyn Dang, Abigail N. VandeWalle, Nicholas A. Peppas, and Marissa E. Wechsler
- Subjects
Carboxylic acid ,Static Electricity ,Nanogels ,Lactoglobulins ,Aldehyde ,Article ,Catalysis ,Protein–protein interaction ,chemistry.chemical_compound ,Polymethacrylic Acids ,Albumins ,Materials Chemistry ,Isoelectric Point ,Carboxylate ,chemistry.chemical_classification ,Acrylamides ,Chemistry ,Metals and Alloys ,General Chemistry ,Combinatorial chemistry ,Surfaces, Coatings and Films ,Electronic, Optical and Magnetic Materials ,Lactoferrin ,Isoelectric point ,Covalent bond ,Immunoglobulin G ,Ceramics and Composites ,Muramidase ,Adsorption ,Protein adsorption ,Nanogel - Abstract
An aldehyde acrylate-based functional monomer was incorporated into poly(N-isopropylacrylamide-co-methacrylic acid) nanogels for use as protein receptors. The aldehyde component forms dynamic imines with surface exposed lysine residues, while carboxylic acid/ carboxylate moieties form electrostatic interactions with high isoelectric point proteins. Together, these interactions effect protein adsorption and recognition.
- Published
- 2020