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Your search keyword '"Mian Zi Tee"' showing total 2 results
Start Over Author "Mian Zi Tee" Topic albendazole
2 results on '"Mian Zi Tee"'

1. Gut microbiome of helminth-infected indigenous Malaysians is context dependent

Author

Mian Zi Tee, Yi Xian Er, Alice V. Easton, Nan Jiun Yap, Ii Li Lee, Joseph Devlin, Ze Chen, Kee Seong Ng, Poorani Subramanian, Angelina Angelova, Oyebola Oyesola, Shushan Sargsian, Romano Ngui, Daniel P. Beiting, Christopher Chiong Meng Boey, Kek Heng Chua, Ken Cadwell, Yvonne Ai Lian Lim, P’ng Loke, and Soo Ching Lee

Subjects
Helminth, Microbiome, Metagenomic sequencing, Indigenous population, Albendazole, Microbial ecology, QR100-130
Abstract

Abstract Background While microbiomes in industrialized societies are well characterized, indigenous populations with traditional lifestyles have microbiomes that are more akin to those of ancient humans. However, metagenomic data in these populations remains scarce, and the association with soil-transmitted helminth infection status is unclear. Here, we sequenced 650 metagenomes of indigenous Malaysians from five villages with different prevalence of helminth infections. Results Individuals from villages with higher prevalences of helminth infections have more unmapped reads and greater microbial diversity. Microbial community diversity and composition were most strongly associated with different villages and the effects of helminth infection status on the microbiome varies by village. Longitudinal changes in the microbiome in response to albendazole anthelmintic treatment were observed in both helminth infected and uninfected individuals. Inference of bacterial population replication rates from origin of replication analysis identified specific replicating taxa associated with helminth infection. Conclusions Our results indicate that helminth effects on the microbiota were highly dependent on context, and effects of albendazole on the microbiota can be confounding for the interpretation of deworming studies. Furthermore, a substantial quantity of the microbiome remains unannotated, and this large dataset from an indigenous population associated with helminth infections is a valuable resource for future studies. Video Abstract

Published
2022
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2. Efficacy of triple dose albendazole treatment for soil-transmitted helminth infections

Author

Mian Zi Tee, Soo Ching Lee, Yi Xian Er, Nan Jiun Yap, Romano Ngui, Alice V. Easton, Vinnie Wei Yin Siow, Kee Seong Ng, Christopher Chiong Meng Boey, Kek Heng Chua, Ken Cadwell, P’ng Loke, and Yvonne Ai Lian Lim

Subjects
Anthelmintics, Ascariasis, Multidisciplinary, Adolescent, Helminthiasis, Albendazole, Feces, Hookworm Infections, Soil, Cross-Sectional Studies, Trichuris, Animals, Humans, Longitudinal Studies, Trichuriasis, Ascaris lumbricoides, Child
Abstract

In Malaysia, soil-transmitted helminth (STH) infections still persist among indigenous communities. In the past, local studies have focused mostly on epidemiologic aspects of STH infections with a scarcity of information on the efficacy of deworming treatment. The present study consisted of 2 phases: a cross-sectional phase on current epidemiological status and risk factors of STH infections and a longitudinal study over 6 weeks on triple dose albendazole efficacy against STH infections. A total of 253 participants were recruited at baseline and a pre-tested questionnaire was administered to obtain information on socio-demographics, environmental and behavioural risk factors. Stool samples were evaluated using a modified Kato-Katz technique. Cure rate (CR) and egg reduction rate (ERR) were assessed at 3 weeks following a 3-day course of 400mg albendazole treatment and infection status were observed again at 6 weeks. Baseline positivity of trichuriasis, ascariasis and hookworm infections were 56.1%, 11.9% and 20.2%, respectively. Multivariate analysis showed age below 18 years old (P = 0.004), without latrine in house (P = 0.042) and indiscriminate defecation (P = 0.032) were associated with STH infections. In the longitudinal study (N = 89), CR for trichuriasis was 64.6%, while CR of 100% was observed for both ascariasis and hookworm. ERR was above 90% for all three STH species. A rapid increased of Trichuris trichiura egg output was observed at 6 weeks. In conclusion, STH infections are highly prevalent among indigenous communities. Children and teenagers, poor sanitation and hygiene behaviour were determinants for STH infections. Triple dose albendazole is found to be efficacious against Ascaris lumbricoides and hookworm infections but has moderate curative effect with high ERR against T. trichiura. Although triple dose albendazole regimen has logistic challenges and may not be a routine option, consideration of this treatment regime may still be necessary in selective communities to reduce high intensity of T. trichiura infection.

Published
2022

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