1. [Immunogenicity of plasmid DNA and adenoviral vectors encoding HIV-1 subtype B env gene].
- Author
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Yang HR, Zhang LF, Feng X, Yu SQ, Zhuang ZL, Li HX, and Zeng Y
- Subjects
- AIDS Vaccines genetics, Adenoviridae genetics, Animals, China, Genetic Vectors genetics, HIV Antibodies genetics, HIV Antibodies immunology, HIV Envelope Protein gp120 genetics, HIV Envelope Protein gp120 immunology, HIV Envelope Protein gp160 genetics, HIV Envelope Protein gp160 immunology, HIV-1 genetics, Mice, Mice, Inbred BALB C, Plasmids genetics, Vaccines, DNA genetics, Vaccines, Synthetic genetics, Vaccines, Synthetic immunology, AIDS Vaccines immunology, Adenoviridae immunology, Genes, env immunology, Genetic Vectors immunology, HIV-1 immunology, Plasmids immunology, Vaccines, DNA immunology
- Abstract
Objective: To construct DNA and recombinant adenovirus vector vaccines containing an env gene from the prevalent subtype B strain in China and try to use them for therapeutic and prophylactic vaccines., Methods: The candidate plasmid DNA vaccine pVR-gp160 and recombinant adenovirus vaccine rAdV-gp160 were constructed separately. BALB/c mice were immunized with these two vaccines in different administration schemes. HIV-1 Gp120-specific cellular responses and antibody levels were detected by ELISPOT and ELISA respectively., Results: DNA vaccine alone and combined vaccines in a DNA prime/rAdV-gp160 boost vaccination regimen induced high level of Gp120-specific cellular responses. While low level of Gp120-specific antibodies were elicited in all groups., Conclusion: DNA and rAdV vaccines could efficiently express Gp160 protein and activate specific cellular responses.
- Published
- 2010