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Sesame (Sesamum indicum L.) is being considered for commercial production in the southeastern USA, but very little is known about its potential and the appropriate management practices required to achieve sustainable yield potential in the area. This study thus aims to determine the optimal planting date and row spacing for several sesame cultivars. Traits examined were yield, plant height, number of nodes on the main stem, number of branches, height to first capsule on the main stem, and number of plants per meter. A first trial investigated three planting dates in Citra, FL (May, June, and July); whereas a second one examined several row spacings at two locations: 19 and 38.1 cm in Citra; and 19, 38.1, and 76.2 cm in Live Oak, FL. The May planting date showed higher yields, number of nodes, and taller plants than the other dates. Accumulated air and soil growing degree days (GDDs), rainfall, and solar radiation values were calculated for each planting date in both years. Soil GDDs provided similar results to air GDDs; thus, either model would seem appropriate for predicting crop development in this region. Plant height, number of nodes with capsules, and number of branches were increased at wider row spacings in the Live Oak trials as compared with narrow rows. These two trials illustrated the potential of sesame for attaining economically sustainable yields in the southeastern USA, but further exploration and modeling of agronomic traits will be needed to reach the crop’s full yield potential in these regions. [ABSTRACT FROM AUTHOR]