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Escherichia coli is a gram-negative bacterium, an intestinal pathogen that can cause intestinal inflammation. Antimicrobial peptides are a class of small molecule peptides, which has good antibacterial activity against a variety of gram-positive and negative bacteria. In this regard, the authors aimed to study the effect of the antimicrobial peptide MPX, which was administered orally, on the intestinal wall of mice infected with the intestinal barrier function, which were infected with E. coli. Synthesis and purification of the antimicrobial peptide MPX (H-INWKGIAAMAKKLL-NH2) was performed by Jier Sheng Hua (Shanghai, China). Mass spectrometry and liquid chromatography (HPLC) were used for this purpose. Escherichia Coli (O157: H7 ATCC43889) was isolated from human faeces in which haemolytic uremic syndrome was reported. Solid LB agar (Solarbio, China) was used to isolate enterohemorrhagic E. coli. The culture was obtained from the Chinese Institute of Veterinary Drug Control (Beijing, China). The results of necropsy found that using of an orally administered MPX could alleviate the damage of E. coli to the liver, spleen, and the lungs were less affected. According to H&E results in case of an orally administered MPX group considerably relieved duodenum and organs on day 7 and day 28. qRT-PCR results showed orally administered MPX could reduce the inflammation-related factors in the mRNA expression of IL-2 and IL-6 and TNF-α on day 7 and day 28. In addition, orally administered MPX could significantly increase them RNA expression of tight junction proteins Occludin and Zo-1 on day 7 and day 28. The results of immune histochemistry further showed that an orally administered MPX could increase the mRNA expression of MUC2 in jejunum. The above results showed that orally administered MPX could alleviate the attack of E. coli on the intestinal tract of mice, relieve intestinal inflammation, and improve the intestinal barrier function. This study lays a theoretical foundation for adding antimicrobial peptides to food. In orally administered MPX authors can see reducing the mRNA expression of inflammation-related factors, thereby alleviating the intestinal inflammation caused by E. coli infection in mice. Authors can add that orally administered MPX shows an increase in mRNA expression of tight junction protein in intestines and improves the intestinal barrier function. This study lays the foundation for adding antimicrobial peptides to food to relieve inflammation and improve barrier function in clinical practices

The article provides the results of studies conducted in 2017 on investigating the effect of the herbal extract of herbs (R.carthamoides, S. coronata, F. ulmaria) on the indices of the clinical condition (behavior, blood, internal organs) of non-linear white mice of both sexes with live weight 30 ± 1.0 g at the age of 3 months. The animals were divided into one control and three experimental groups of 15 mice each. The phytoextract dissolved in physiological solution was administered orally daily to the mice of the experimental groups for 7 days in doses: the first group 0.1 ml (0.65 mg of extractive substances), the second group 0.3 ml (1.95 mg) and the third group 0.5 ml (3.25 mg). In control - physiological solution in a dose of 0.5 ml. During the experiment, the general condition of the animals was observed. Before and after the experiment, the mice were weighed. When the experiment was completed, the mice were taken blood for a number of morphological and biochemical studies, including characterizing and antioxidant status. The effect of internal organs (liver, kidneys, spleen) on the mass was evaluated. The results of blood tests showed that in mice of the second experimental group, the number of erythrocytes in comparison with the control (4.2 x1012 kl / l) increased by 45% (6.1 x 1012kl / l), hemoglobin - by 6% (from 112 up to 119 g / l); total protein in serum by 7% (from 61.2 to 65.5 g / l), albumin - by 4% (from 73.5 to 76.5%), SH-group of proteins - by 54% (from 2,2 to 3.4 mmol / l), а-globulins decreased by 39% (from 14.2 to 8.7%), malonic dialdehyde - by 45% (from 6.3 to 3.5 μmol / l) ( P 0.05) differences and was within the regulatory limits.

The aim of the research was to study the chemical composition and toxic properties of plant phytocomplex, which have the most pronounced adaptogenic properties. Object of the study: a phytocomplex including levsea safflower (Rhaponticum carthamoides), serpent crown (Serratula coronata), fenugreev creeper (Filipendula ulmaria). Initially, the chemical composition was determined in selected plants, and then the alcohol extract from the phytocomplex was tested in white mice for toxicity. The toxicity study of extract of the studied phytocomplex was carried out on mice divided into 2 experiment and 2 control groups (n = 5 in each group), which was injected parenterally and intragastrically at once in a volume of 0.5 ml/mouse; the maximum dose of extractive substances of the phytocomplex was 2.5 g/kg of body weight in terms of dry matter. In the control, physiological saline was injected once in the same volume. Within 10 days the clinical condition of the animals was monitored. Before and after the experiment the blood was examined for the number of erythrocytes, leukocytes and hemoglobin concentration. The results of studies of phytocomplex containing water-alcohol extracts of the herbs Rhaponticum carthamoides, Serratula coronata, Filipendula ulmaria, showed that the biologically active substances in it are: amino acids - aspartic (3.5.3.9 g/100 g), glutamic (2,5...3.0 g /100 g), leucine (1.9...2.0 g/100 g), tyrosine (1.2...1.4 g/100 g), lysine (1.6 g /100 g ); Vitamins C (62.0.77.0 mg /%), E (6.2 mg /%), carotenoids (65.0.113.0 mg /%), PP (11.5 mg /% ); Ecdysteroids (418.0.2170 mg / kg), and flavonoids (3.0.. .7.2% of the dry matter). All the herbs of the phytocomplex contain protein, sugars, vitamins, amino acids, tannins and flavonoids. Intragastric and intraperitoneal administration of the phytocomplex to white mice showed no toxic properties.

The paper presents the research materials on the influence of the phytobakterial complex FANTPLUS, including the microorganism Lactobacillus plantarum and Leuzea carthamoides D.C., on the clinical state (blood, behavioral responses, internal organs) of non-linear white mice aged 90 days. The experiment was carried out for 30 days on 25 white mice, divided into one control and 4 test groups of 5 mice each. Daily in the diet of mice of experimental groups FANTPLUS was administered at the rate of gram per mouse per day: the first one was 0.1; The second - 0.2; The third - 0.3; The fourth - 0.4. Throughout the observation period, the mice of all groups did not show abnormalities in the clinical state. The increase in body weight in comparison with baseline in the first trial group was 8.9%, in the second group - 11.3%, in the third group - 20.8%, in the fourth group - 18.0%, in control - 7.8% %. Pathologies from the internal organs (liver, kidneys, spleen) have not been revealed. Changes in body mass index, internal organs and blood occurred within the physiological norm for mice of this age group. The content of leukocytes increased by 11-24%, erythrocytes - by 12-28%, lymphocytes - by 11-21%; The relative distribution of erythrocytes by volume - by 22-62%. The concentration of hemoglobin increased by 5.8-15%, the average concentration of hemoglobin in erythrocytes by 2-10%, hematocrit increased in all test groups from 5 to 29%, the number of platelets by 4-6%, thrombocrit by 17-23%. The coefficient of large platelets increased by 18-40%. The Streeter index increased by 7-44%, and the opsonophagocytic index (OFI) by 12-53% in comparison with the control. Phytoplasmic phantom bacteria complex FANTPLYUS in a dose of 0.3 g / kg body weight of mice optimized the studied blood indices, increasing, in comparison with the control, the number of leukocytes, erythrocytes, platelets, hemoglobin, hematocrit and thrombocrit. Similarly, the phytobacterial complex also influenced the immunological parameters, which was manifested by the intensification of the opsonophagocytic reaction.