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Early and accurate disease diagnosis is pivotal for effective phytosanitary management strategies in agriculture. Hyperspectral sensing has emerged as a promising tool for early disease detection, yet challenges remain in effectively harnessing its potential. This study compares parametric spectral Vegetation Indices (VIs) and a nonparametric Gaussian Process Classification based on an Automated Spectral Band Analysis Tool (GPC-BAT) for diagnosing plant bacterial diseases using hyperspectral data. The study conducted experiments on tomato plants in controlled conditions and kiwi plants in field settings to assess the performance of VIs and GPC-BAT. In the tomato experiment, the modeling processes were applied to classify the spectral data measured on the healthy class of plants (sprayed with water only) and discriminate them from the data captured on plants inoculated with the two bacterial suspensions (108 CFU mL−1). In the kiwi experiment, the standard modeling results of the spectral data collected on nonsymptomatic plants were compared to the ones obtained using symptomatic plants' spectral data. VIs, known for their simplicity in extracting biophysical information, successfully distinguished healthy and diseased tissues in both plant species. The overall accuracy achieved was 63% and 71% for tomato and kiwi, respectively. Limitations were observed, particularly in differentiating specific disease infections accurately. On the other hand, GPC-BAT, after feature reduction, showcased enhanced accuracy in identifying healthy and diseased tissues. The overall accuracy ranged from 70% to 75% in the tomato and kiwi case studies. Despite its effectiveness, the model faced challenges in accurately predicting certain disease infections, especially in the early stages. Comparative analysis revealed commonalities and differences in the spectral bands identified by both approaches, with overlaps in critical regions across plant species. Notably, these spectral regions corresponded to the absorption regions of various photosynthetic pigments and structural components affected by bacterial infections in plant leaves. The study underscores the potential of hyperspectral sensing in disease diagnosis and highlights the strengths and limitations of VIs and GPC-BAT. The identified spectral features hold biological significance, suggesting correlations between bacterial infections and alterations in plant pigments and structural components. Future research avenues could focus on refining these approaches for improved accuracy in diagnosing diverse plant–pathogen interactions, thereby aiding disease diagnosis. Specifically, efforts could be directed towards adapting these methodologies for early detection, even before symptom manifestation, to better manage agricultural diseases. [ABSTRACT FROM AUTHOR]

Plant-soil sensing devices coupled with Artificial Intelligence autonomously collect and process in situ plant phenotypic data. A challenge of this approach is the limited incorporation of phenotype data into decision support systems designed to harness agricultural practices and forecast plant behavior within the intricate context of genotype, environment, and management interactions (G × E × M). To enhance the role of digital phenotyping in supporting Precision Agriculture, this paper proposes a sensing network based on the Internet of Things. The developed system comprises three modules: data collection, communication, and a cloud server. Several processes co-occur in the server, namely data visualization to confirm the correct sensors and data stream functioning. In addition, a crop growth model (CGM) runs on the server, which is powered by the collected data. The simulations generated by the model will support agricultural decisions, obtaining, in advance, insights about plant behavior considering several G × E × M scenarios. To assess the performance of the proposed network to provide reliable data to the model, a greenhouse was equipped with several sensors that collect plant, environment, and soil data (e.g., leaf numbers, air temperature, soil moisture). The proposed network can provide real-time causal support for advanced agricultural practices, evolving from a data-driven approach to an integrative framework where context (G × E × M) drives decision making. [ABSTRACT FROM AUTHOR]

Robotics in agriculture faces several challenges, such as the unstructured characteristics of the environments, variability of luminosity conditions for perception systems, and vast field extensions. To implement autonomous navigation systems in these conditions, robots should be able to operate during large periods and travel long trajectories. For this reason, it is essential that simultaneous localization and mapping algorithms can perform in large‐scale and long‐term operating conditions. One of the main challenges for these methods is maintaining low memory resources while mapping extensive environments. This work tackles this issue, proposing a localization and mapping approach called VineSLAM that uses a topological mapping architecture to manage the memory resources required by the algorithm. This topological map is a graph‐based structure where each node is agnostic to the type of data stored, enabling the creation of a multilayer mapping procedure. Also, a localization algorithm is implemented, which interacts with the topological map to perform access and search operations. Results show that our approach is aligned with the state‐of‐the‐art regarding localization precision, being able to compute the robot pose in long and challenging trajectories in agriculture. In addition, we prove that the topological approach innovates the state‐of‐the‐art memory management. The proposed algorithm requires less memory than the other benchmarked algorithms, and can maintain a constant memory allocation during the entire operation. This consists of a significant innovation, since our approach opens the possibility for the deployment of complex 3D SLAM algorithms in real‐world applications without scale restrictions. [ABSTRACT FROM AUTHOR]

Currently, Unmanned Aerial Vehicles (UAVs) are considered in the development of various applications in agriculture, which has led to the expansion of the agricultural UAV market. However, Nano Aerial Vehicles (NAVs) are still underutilised in agriculture. NAVs are characterised by a maximum wing length of 15 centimetres and a weight of fewer than 50 g. Due to their physical characteristics, NAVs have the advantage of being able to approach and perform tasks with more precision than conventional UAVs, making them suitable for precision agriculture. This work aims to contribute to an open-source solution known as Nano Aerial Bee (NAB) to enable further research and development on the use of NAVs in an agricultural context. The purpose of NAB is to mimic and assist bees in the context of pollination. We designed this open-source solution by taking into account the existing state-of-the-art solution and the requirements of pollination activities. This paper presents the relevant background and work carried out in this area by analysing papers on the topic of NAVs. The development of this prototype is rather complex given the interactions between the different hardware components and the need to achieve autonomous flight capable of pollination. We adequately describe and discuss these challenges in this work. Besides the open-source NAB solution, we train three different versions of YOLO (YOLOv5, YOLOv7, and YOLOR) on an original dataset (Flower Detection Dataset) containing 206 images of a group of eight flowers and a public dataset (TensorFlow Flower Dataset), which must be annotated (TensorFlow Flower Detection Dataset). The results of the models trained on the Flower Detection Dataset are shown to be satisfactory, with YOLOv7 and YOLOR achieving the best performance, with 98% precision, 99% recall, and 98% F1 score. The performance of these models is evaluated using the TensorFlow Flower Detection Dataset to test their robustness. The three YOLO models are also trained on the TensorFlow Flower Detection Dataset to better understand the results. In this case, YOLOR is shown to obtain the most promising results, with 84% precision, 80% recall, and 82% F1 score. The results obtained using the Flower Detection Dataset are used for NAB guidance for the detection of the relative position in an image, which defines the NAB execute command. [ABSTRACT FROM AUTHOR]

The efficiency of agricultural practices depends on the timing of their execution. Environmental conditions, such as rainfall, and crop-related traits, such as plant phenology, determine the success of practices such as irrigation. Moreover, plant phenology, the seasonal timing of biological events (e.g., cotyledon emergence), is strongly influenced by genetic, environmental, and management conditions. Therefore, assessing the timing the of crops' phenological events and their spatiotemporal variability can improve decision making, allowing the thorough planning and timely execution of agricultural operations. Conventional techniques for crop phenology monitoring, such as field observations, can be prone to error, labour-intensive, and inefficient, particularly for crops with rapid growth and not very defined phenophases, such as vegetable crops. Thus, developing an accurate phenology monitoring system for vegetable crops is an important step towards sustainable practices. This paper evaluates the ability of computer vision (CV) techniques coupled with deep learning (DL) (CV_DL) as tools for the dynamic phenological classification of multiple vegetable crops at the subfield level, i.e., within the plot. Three DL models from the Single Shot Multibox Detector (SSD) architecture (SSD Inception v2, SSD MobileNet v2, and SSD ResNet 50) and one from You Only Look Once (YOLO) architecture (YOLO v4) were benchmarked through a custom dataset containing images of eight vegetable crops between emergence and harvest. The proposed benchmark includes the individual pairing of each model with the images of each crop. On average, YOLO v4 performed better than the SSD models, reaching an F1-Score of 85.5%, a mean average precision of 79.9%, and a balanced accuracy of 87.0%. In addition, YOLO v4 was tested with all available data approaching a real mixed cropping system. Hence, the same model can classify multiple vegetable crops across the growing season, allowing the accurate mapping of phenological dynamics. This study is the first to evaluate the potential of CV_DL for vegetable crops' phenological research, a pivotal step towards automating decision support systems for precision horticulture. [ABSTRACT FROM AUTHOR]

Effects of glucose availability were investigated in Lactobacillus sakei strains 23K and LS25 cultivated in anaerobic, glucose-limited chemostats set at high (D = 0.357 h-1) and low (D = 0.045 h-1) dilution rates. We observed for both strains a shift from homolactic towards more mixed acid fermentation when comparing high to low growth rates. However, this change was more pronounced for LS25 than for 23K, where dominating products were lactate>formate>acetate≥ethanol at both conditions. A multivariate approach was used for analyzing proteome and transcriptome data from the bacterial cultures, where the predictive power of the omics data was used for identifying features that can explain the differences in the end-product profiles. We show that the different degree of response to the same energy restriction revealed interesting strain specific regulation. An elevated formate production level during slow growth, more for LS25 than for 23K, was clearly reflected in correlating pyruvate formate lyase expression. With stronger effect for LS25, differential expression of the Rex transcriptional regulator and NADH oxidase, a target of Rex, indicated that maintainance of the cell redox balance, in terms of the NADH/NAD+ ratio, may be a key process during the metabolic change. The results provide a better understanding of different strategies that cells may deploy in response to changes in substrate availability. [ABSTRACT FROM AUTHOR]

The agricultural sector plays a fundamental role in our society, where it is increasingly important to automate processes, which can generate beneficial impacts in the productivity and quality of products. Perception and computer vision approaches can be fundamental in the implementation of robotics in agriculture. In particular, deep learning can be used for image classification or object detection, endowing machines with the capability to perform operations in the agriculture context. In this work, deep learning was used for the detection of grape bunches in vineyards considering different growth stages: the early stage just after the bloom and the medium stage where the grape bunches present an intermediate development. Two state-of-the-art single-shot multibox models were trained, quantized, and deployed in a low-cost and low-power hardware device, a Tensor Processing Unit. The training input was a novel and publicly available dataset proposed in this work. This dataset contains 1929 images and respective annotations of grape bunches at two different growth stages, captured by different cameras in several illumination conditions. The models were benchmarked and characterized considering the variation of two different parameters: the confidence score and the intersection over union threshold. The results showed that the deployed models could detect grape bunches in images with a medium average precision up to 66.96%. Since this approach uses low resources, a low-cost and low-power hardware device that requires simplified models with 8 bit quantization, the obtained performance was satisfactory. Experiments also demonstrated that the models performed better in identifying grape bunches at the medium growth stage, in comparison with grape bunches present in the vineyard after the bloom, since the second class represents smaller grape bunches, with a color and texture more similar to the surrounding foliage, which complicates their detection. [ABSTRACT FROM AUTHOR]

The development of robotic solutions in unstructured environments brings several challenges, mainly in developing safe and reliable navigation solutions. Agricultural environments are particularly unstructured and, therefore, challenging to the implementation of robotics. An example of this is the mountain vineyards, built-in steep slope hills, which are characterized by satellite signal blockage, terrain irregularities, harsh ground inclinations, and others. All of these factors impose the implementation of precise and reliable navigation algorithms, so that robots can operate safely. This work proposes the detection of semantic natural landmarks that are to be used in Simultaneous Localization and Mapping algorithms. Thus, Deep Learning models were trained and deployed to detect vine trunks. As significant contributions, we made available a novel vine trunk dataset, called VineSet, which was constituted by more than 9000 images and respective annotations for each trunk. VineSet was used to train state-of-the-art Single Shot Multibox Detector models. Additionally, we deployed these models in an Edge-AI fashion and achieve high frame rate execution. Finally, an assisted annotation tool was proposed to make the process of dataset building easier and improve models incrementally. The experiments show that our trained models can detect trunks with an Average Precision up to 84.16% and our assisted annotation tool facilitates the annotation process, even in other areas of agriculture, such as orchards and forests. Additional experiments were performed, where the impact of the amount of training data and the comparison between using Transfer Learning and training from scratch were evaluated. In these cases, some theoretical assumptions were verified. [ABSTRACT FROM AUTHOR]

Research and development of autonomous mobile robotic solutions that can perform several active agricultural tasks (pruning, harvesting, mowing) have been growing. Robots are now used for a variety of tasks such as planting, harvesting, environmental monitoring, supply of water and nutrients, and others. To do so, robots need to be able to perform online localization and, if desired, mapping. The most used approach for localization in agricultural applications is based in standalone Global Navigation Satellite System-based systems. However, in many agricultural and forest environments, satellite signals are unavailable or inaccurate, which leads to the need of advanced solutions independent from these signals. Approaches like simultaneous localization and mapping and visual odometry are the most promising solutions to increase localization reliability and availability. This work leads to the main conclusion that, few methods can achieve simultaneously the desired goals of scalability, availability, and accuracy, due to the challenges imposed by these harsh environments. In the near future, novel contributions to this field are expected that will help one to achieve the desired goals, with the development of more advanced techniques, based on 3D localization, and semantic and topological mapping. In this context, this work proposes an analysis of the current state-of-the-art of localization and mapping approaches in agriculture and forest environments. Additionally, an overview about the available datasets to develop and test these approaches is performed. Finally, a critical analysis of this research field is done, with the characterization of the literature using a variety of metrics. [ABSTRACT FROM AUTHOR]