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Start Over Author "Guillén-Sánchez, Dagoberto" Topic agriculture
2 results on '"Guillén-Sánchez, Dagoberto"'

1. Detección del virus de la hoja amarilla de la caña de azúcar y virus mosaico de la caña de azúcar en sorgo (Sorghum bicolor (L.) Moench) en el Estado de Morelos, México

Author

Perales-Rosas, Daniel, Hernández-Pérez, Ricardo, Guillén-Sánchez, Dagoberto, López-Martínez, Víctor, Alia-Tejacal, Irán, Andrade-Rodríguez, María, Juárez-López, Porfirio, and Perdomo-Roldán, Francisco

Subjects
sorgo, Agriculture (General), DAS-ELISA, Agriculture, sorghum, virus, S1-972
Abstract

El presente estudio se realizó en los meses de junio-julio del 2017, con el propósito de determinar la presencia de virus asociados al cultivo del sorgo (Sorghum bicolor (L.) Moench). Se muestrearon 21 localidades, distribuidas en 10 municipios de los 33 que forman el Estado de Morelos. Se utilizaron dos anticuerpos para detectar las enfermedades Virus de la hoja amarilla de la caña de azúcar (SCYLV) y Virus mosaico de la caña de azúcar (SCMV). Se analizaron muestras de follaje colectadas al azar en cinco puntos de cada campo o parcela. El diagnóstico se realizó mediante el test inmunoenzimático tipo DAS-ELISA (doble anticuerpo), con un set de reactivos AGDIA (2017), con conjugado de fosfatasa alcalina para (SCMV) y el segundo set de reactivos de NANO Diagnostic (2017) "AC Diagnostic" con conjugado fosfatasa alcalina para (SCYLV). Los resultados obtenidos evidenciaron presencia de ambas enfermedades virales en el cultivo del sorgo para el estado de Morelos. Con muestras positivas al (SCYLV) y absorbancias altas (1,50 A° y 1,10 A°) en los municipios de Amacuzac y Tequesquitengo respectivamente. Mientras solo dos muestras positivas al (SCMV) fueron detectadas en las localidades de Puente de Ixtla y Yautepec, pero con valores bajos de absorbancia respecto a los controles positivos The study was carried out in the months of June-July 2017, with the purpose of studying the current situation of the viruses associated with the cultivation of sorghum (Sorghum bicolor (L.) Moench). Twenty-one commercial sorghum plantation in Morelos state, were analyzed to detected Sugarcane yellow leaf virus (SCYLV) and Sugarcane mosaic virus (SCMV). The diagnosis was made, by means DAS-ELISA (double antibody) an immunoenzymatic test, that used a AGDIA kit (2017) and a reagent set of NANO Diagnostic (2017) "AC Diagnostic" with conjugated alkaline phosphatase, to detection of SCMV and SCYLV respectively. The results obtained evidenced, the presence of both viral diseases in the sorghum cultivation, in ten municipalities of Morelos state whit predominance of SCYLV. Positives samples to this virus with high absorbance (1.50 A° and 1.10 A°) on the municipalities of Amacuzac and Tequesquitengo, respectively were observed. While, only two positive samples of SCMV, in Puente de Ixtla and Yautepec were detected, but with low absorbance values respect to positive controls

Published
2018

2. Vanadium stimulates pepper plant growth and flowering, increases concentrations of amino acids, sugars and chlorophylls, and modifies nutrient concentrations.

Author

García-Jiménez, Atonaltzin, Trejo-Téllez, Libia Iris, Guillén-Sánchez, Dagoberto, and Gómez-Merino, Fernando Carlos

Subjects
PHYSIOLOGICAL effects of vanadium, PLANT nutrients, PLANT growth, AMINO acids, BLACK pepper (Plant), CHLOROPHYLL
Abstract

Vanadium (V) can be absorbed by plants and regulate their growth and development, although contrasting effects have been reported among species and handling conditions. The objective of this work was to evaluate the beneficial effect of V on pepper plants (Capsicum annuum L.). The plants were grown in a hydroponic system with the application of four V concentrations (0, 5, 10, and 15 μM NH₄VO₃). Four weeks after the beginning of the treatments, growth, flowering, biomass, chlorophyll concentration, total amino acids, total soluble sugars, and nutrients were determined in leaves, stems, and roots. The application of 5 μM V increased plant growth, induced floral bud development, and accelerated flowering. The chlorophyll concentration varied according to the type of plant part analyzed. The concentrations of amino acids and sugars in leaves and roots were higher with 5 μM. With 10 and 15 μM V, the plants were smaller and showed toxicity symptoms. The K concentration in leaves decreased as the V dose increased (0 to 15 μM). However, 5 μM V increased the concentrations of N, P, K, Ca, Mg, Cu, Mn, and B, exclusively in stems. The application of 15 μM V decreased the concentrations of Mg and Mn in leaves, but increased those of P, Ca, Mg, Cu, and B in roots. We conclude that V has positive effects on pepper growth and development, as well as on the concentrations of amino acids and total sugars. V was antagonistic with K, Mg, and Mn in leaves, while in stems and roots, there was synergism with macro and micronutrients. Vanadium is a beneficial element with the potential to be used in biostimulation approaches of crops like pepper. [ABSTRACT FROM AUTHOR]

Published
2018
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