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The patterns of plant species diversity in herbaceous vegetation subjected to various human activities were studied in most of the landscape elements in a rural area of Chiba, central Japan. Twenty-eight transects were sampled in four types of human management-regime (cultivation, trampling, mowing, and abandonment) and were grouped into seven vegetation types using TWINSPAN and DCA analyses. The DCA axis 1 arranged all the transects into a successional order along which biomass and the degree of succession increased. Accumulated number of species increased in a stepwise pattern along the DCA axis 1, in which the dominant plant life-forms were replaced from annuals, to perennials and perennials/tree-saplings depending on different management regimes. The unique species which were confined to a certain management regime, were identified in each site. It is suggested that the coexistence of various successional communities under different human management regimes enhance regional species diversity through maintaining these unique species. Among four types of management regime, mowing sites (especially traditional verge meadows) had most abundant unique, rare species specially adapted to regular cutting. It is suggested that maintaining such traditional mown sites is important to conserve the unique biodiversity of the studied area. [Copyright &y& Elsevier]