1. Study on characterization of Bupleurum chinense polysaccharides with antioxidant mechanisms focus on ROS relative signaling pathways and anti-aging evaluation in vivo model.
- Author
-
Feng, Anqi, Zhao, Ziwei, Liu, Changfeng, Du, Cheng, Gao, Pinyi, Liu, Xuegui, and Li, Danqi
- Subjects
- *
CELLULAR signal transduction , *BUPLEURUM , *AGING prevention , *POLYSACCHARIDES , *DROSOPHILA melanogaster , *PI3K/AKT pathway , *MONOSACCHARIDES - Abstract
This study explored the structures of three polysaccharides from Bupleurum chinense DC. (BCPRs), and evaluated their antioxidant and anti-aging properties. The HPGPC and ion chromatography analyses revealed that the molecular weights of the BCPRs ranged from 12.05 to 21.20 kDa, and were primarily composed of rhamnose, arabinose, xylose, galactose, glucose and galacturonic acid. Methylation and NMR studies identified 10 PMAAs, establishing the various backbones of BCPRs 1–3. BCPR-3 demonstrated potent antioxidant activities, including DPPH, ABTS, hydroxy, and superoxide radicals scavenging in vitro. At concentrations between 125 and 500 μg/mL, BCPR-3 increased T-AOC, SOD and GSH-Px activities, while decreasing MDA levels in H 2 O 2 -induced SH-SY5Y cells. In addition, RNA-seq results indicated that BCPR-3 considerably downregulated the expression of 49 genes and upregulated five genes compared with the control group. KEGG analysis suggested that these differentially expressed genes (DEGs) were predominantly involved in the TNF and PI3K/Akt signaling pathways. Furthermore, in vivo experiment with Drosophila melanogaster showed that BCPR-3 could extend the average lifespan of flies. In conclusion, polysaccharides from B. chinense exhibited potential antioxidant and anti-aging activities, which could be developed as new ingredients to combat oxidative stress damage and slow the aging process. [Display omitted] • Three acid polysaccharides were isolated from the roots of Bupleurum chinense DC. • BCPR-1, BCPR-2 and BCPR-3 possessed antioxidant activities. • BCPR-3 had a protective effect on SH-SY5Y cell injury induced by H 2 O 2. • BCPR-3 exerts antioxidant effect by regulating TNF and PI3K/Akt signaling pathways. • BCPR-3 could relatively prolong the life span of Drosophila melanogaster. [ABSTRACT FROM AUTHOR]
- Published
- 2024
- Full Text
- View/download PDF