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The adenosine-producing ectoenzyme 5'-nucleotidase has recently been shown to undergo a marked redistribution during development of the cat visual cortex and to be involved in the remodelling of ocular dominance columns (Schoen et al., J. Comp. Neurol., 296, 379-392, 1990). Using an enzyme-cytochemical technique, we now investigate the developmental redistribution of 5'-nucleotidase activity in area 17 of kittens at the ultrastructural level. Between postnatal days 35 and 42, when 5'-nucleotidase is concentrated in layer IV, enzyme reaction product occupies the clefts of asymmetrical synapses within the neuropil. During later development (9th and 13th postnatal weeks), when 5'-nucleotidase spreads over all cortical laminae, the enzyme disappears from its synaptic localization and becomes increasingly associated with astrocytic membranes. The transient appearance of 5'-nucleotidase at synapses parallels the time-course and laminar profile of the synaptic remodelling which takes place during the critical period of visual cortex development. This suggests that synapse-bound 5'-nucleotidase activity plays a role in synaptic malleability, whereas its later association with glial profiles is likely to reflect other functions of the enzyme.

The distribution of the adenosine-producing ecto-enzyme 5'-nucleotidase was investigated histochemically in the visual cortex of normally reared and monocularly deprived kittens and cats. In normally reared kittens aged between 11 to 44 days, 5'-nucleotidase activity formed a band of intense neuropil staining throughout cortical layer IV of areas 17 and 18. The other layers were almost devoid of reaction product. Between the 4th and 6th week, this band had a patchy appearance in area 17, the center-to-center spacing of 5'-nucleotidase patches being approximately 1 mm. Monocular enucleation accentuated these patches of enhanced 5'-nucleotidase activity or made them reappear at developmental stages at which they had normally faded. Simultaneous visualization of ocular dominance columns by transneuronal transport of intraocularly injected 3H-proline showed that the patches of enhanced 5'-nucleotidase activity coincided with the territories of afferents from the intact eye. With increasing age and normal visual development, the patches disappeared and 5'-nucleotidase activity spread to the supra- and infragranular layers. The adult pattern was characterized by dense staining of all cortical laminae in both areas 17 and 18 and was established at about 8 weeks of age. At approximately 7 weeks of age, when the patches in layer IV had disappeared in the course of normal development, monocular enucleation caused a reappearance of the discontinuous pattern of 5'-nucleotidase activity in layer IV. These results reveal a close relation between the distribution of 5'-nucleotidase and the time course of the developmental phase during which the visual cortex is susceptible to experience-dependent alterations. As suggested by the correlation between sites of enzyme activity and eye dominance columns, the expression of 5'-nucleotidase patches in layer IV appears to be associated with the remodelling of ocular dominance territories that occurs both in normal development and after manipulation of afferent retinal input. Thus, 5'-nucleotidase is likely to serve a function in activity-dependent modifications of cortical circuitry. Moreover, 5'-nucleotidase activity is the only endogenous marker known to date that exhibits a columnar pattern in cat visual cortex.

The distribution of the ectoenzyme 5'-nucleotidase was studied in the developing rat cerebellum using enzyme- and immunocytochemical techniques in order to evaluate its appearance in the processes of cellular migration during nervous system development. 5'-Nucleotidase enzymatic activity appeared first in the external granular layer (postnatal day 6). Then, the inner portion of the external granular layer and the developing molecular layer became prominently stained (postnatal day 12). At 21 days the adult pattern was established with a diffuse labeling of the whole molecular layer. Additionally, 5'-nucleotidase enzymatic activity was found around Purkinje cells. At the ultrastructural level migrating nerve cells exhibited an intense surface labeling whereas resting neuroblasts of the external granular layer as well as all post-migratory nerve cells were negative. Immunocytochemistry differed from enzyme histochemistry in that immunoreactivity was found to be selectively associated with glial elements. It is suggested that 5'-nucleotidase may serve as a marker for processes of neuronal migration and neuron-glia interactions during postnatal ontogeny. Underlying mechanisms could be due to an interaction of 5'-nucleotidase with the extracellular matrix molecule, laminin, and cytoskeletal actin involved in locomotion.