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Dengue is an infection of great global importance with different clinical presentations.To establish the seroprevalence of infection by the dengue virus in a district of the Paraguayan Chaco.Cross-sectional study in 418 inhabitants of three villages in the district of Villa Hayes, Paraguay, using a probabilistic household sampling, a questionnaire and blood sampling. Antibodies were determined by the ELISA capture IgG anti dengue method and factors associated with seroprevalence were evaluated.The overall seroprevalence for dengue virus infection was 24,2% (CI95%: 20,2%-28,6%); 34% (n: 142) of respondents reported history of dengue in the previous 10-year period. Of the 276 people (66%) who reported no history of dengue infection, 37 (13%) tested positive, which points out asymptomatic individuals. The main factors associated with infection were: males with significantly higher prevalence than women (31%); to be over 60 years of age (44%), be residents of Villa Hayes (31,1%). No association with educational level or monthly income of the participants, nor housing condition, drinking water source or type of bathroom were observed. The larval infestation rate was 0,51%.The relatively low seroprevalence of infection by the dengue virus is consistent with the small size of the district and highlights the potential risk of infection in future epidemics.

Leptospirosis is a serious infectious disease that has been linked to occupational activities such as farmers, sewer workers, garbage collectors, butchers and veterinarians. It is acquired through contact with major reservoirs like rodents, pets, or the environment contaminated by their urine. In Paraguay, there are no published reports on the seroprevalence of leptospirosis related to work activity.To determine the seroprevalence and factors associated with transmission of leptospirosis in urban sanitation workers.Descriptive transversal study. The study included workers from the Department of Urban Cleanliness of the City of Asuncion, who had completed at least one month on the job and who agreed to particípate in the study. Sampling was stratified and proportional to the number of workers. After informed consent was obtained, participants were surveyed and a blood sample was taken for detection of Leptospira IgG antibodies by ELISA.Leptospirosis seroprevalence was 8.6% (29/339), all positive cases were male and prevalence was statistically higher in workers of the collection area than in those of other work areas.We found a lower than expected seroprevalence. However, conditions observed at work and at home are conducive to transmission of infection. We recommend the improvement of prevention strategies and health promotion in this population.